- Производитель:
- Sigma-Aldrich
eCl@ss: | 32160702 |
NACRES: | NA.41 |
Кат. номер |
AB5535-25UG |
AB5535 |
Specificity
Recognizes Sox9.
Epitope: C-terminal
Transcription Factors
Anti-Sox9 Antibody is a well characterized affinity purified Rabbit Polyclonal Antibody that reliably detects Transcription Factor Sox-9. This highly published antibody has been validated in IHC & WB.
Research Category
Epigenetics & Nuclear Function
Immunohistochemistry Analysis: An 1:1,000 dilution from a representative lot detected Sox9 in murine embryonic bone and adult cartilage tissue sections.
Western Blotting Analysis: An 1:500 dilution from a representative lot detected Sox9 in human PC3 prostate cancer cells and HepG2 hepatocytes.
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Sox9 occupancy at target chromatin sites by ChIP using chromatin preparations from P1 post-natal mouse rib chondrocytes (Ohba, S., et al. (2015). Cell Rep. 12(2):229-243).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Sox9 occupancy at the Bmi promoter in Z/sox9tg but not in wild-type control mouse embryonic fibroblasts/MEFs (Matheu, A., et al. (2012). Cancer Res. 72(5):1301-1315).
ChIP-sequencing (ChIP-seq) Analysis: A representative lot detected Sox9-targeted chromatin sites by a genome-wide ChIP-seq analysis using chromatin preparations from P1 post-natal mouse rib chondrocytes (Ohba, S., et al. (2015). Cell Rep. 12(2):229-243).
Immunofluorescence Analysis: A representative lot detected the accumulation of Sox9-positive oval cells by fluorescent immunohistochemistry staining of paraffin-embedded liver sections from transgenic mice treated with diethylnitrosamine to induce conditional liver HNF4a knockout (Saha, S.K., et al. (2014). Nature. 513(7516):110-114).
Immunofluorescence Analysis: Representative lots detected Sox9 immunoreactivity in paraffin-embedded mouse embryo sections by fluorescent immunohistochemistry (Carrasco, M., et al. (2012). J. Clin. Invest. 122(10):3504-3515; Sylva, M., et al. (2011). PLoS One. 6(8):e22616).
Immunofluorescence Analysis: Representative lots immunostained Muller glial cells in frozen mouse and chicken retina sections by fluorescent immunohistochemistry staining of (Muranishi, Y., and Furukawa, T. (2012). J. Biomed. Biotechnol. 2012:973140; Fischer, A.J., et al. (2011). Neuroscience. 178:250-260).
Immunocytochemistry Analysis: A representative lot detected the stem cell marker Sox9 by fluorescent immunocytochemistry staining of paraformaldehyde-fixed E-Cad/Lgr6+ human lung stem cells (HLSCs) clonally derived and passaged in culture (Oeztuerk-Winder, F., et al. (2012). EMBO J. 31(16):3431-3441).
Immunohistochemistry Analysis: A representative lot immunostained the supporting cells (Sertoli) of the seminiferous tubules by immunohistochemistry staining of paraffin-embedded mouse testis sections (OShaughnessy, P.J., et al. (2012). PLoS One. 7(4):e35136).
Immunohistochemistry Analysis: A representative lot detected Sox9 immunoreactivity in various formalin-fixed, paraffin-embedded human tumor tissue sections (Matheu, A., et al. (2012). Cancer Res. 72(5):1301-1315).
Western Blotting Analysis: A representative lot detected the stem cell marker Sox9 in E-Cad/Lgr6+ human lung stem cells (HLSCs) clonally derived and passaged in culture (Oeztuerk-Winder, F., et al. (2012). EMBO J. 31(16):3431-3441).
Western Blotting Analysis: A representative lot detected upregulated Sox9 expression level in human colorectal cancer cell lines, HCT116, DLD1, and SW620 (Matheu, A., et al. (2012). Cancer Res. 72(5):1301-1315).
Quality
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: An 1:2000 dilution of this antibody detected Sox9 in HepG2 cell lysate.
Target description
56-65 kDa
Physical form
ImmunoAffinity Purified
Purified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide
Storage and Stability
Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
Analysis Note
Control
Embryonic tissue, Adult chondrocytes.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source | rabbit |
Quality Level | 100 |
antibody form | affinity isolated antibody |
antibody product type | primary antibodies |
clone | polyclonal |
purified by | affinity chromatography |
species reactivity | rat, chicken, mouse, human |
species reactivity (predicted by homology) | sheep (based on 100% sequence homology), equine (based on 100% sequence homology), feline (based on 100% sequence homology), bovine (based on 100% sequence homology) |
packaging | antibody small pack of 25 µg |
Торговая марка | Chemicon® |
application(s) | ChIP: suitable (ChIP-seq)","immunocytochemistry: suitable","immunofluorescence: suitable","immunohistochemistry: suitable","western blot: suitable |
NCBI accession no. | NP_000337, |
UniProt accession no. | P48436, |
shipped in | ambient |
storage temp. | 2-8°C |
Gene Information | human ... SOX9(6662), SRA1(10011) |