- Производитель:
- Sigma-Aldrich
Кат. номер |
MABF2793-25UL |
MABF2793-100UL |
Specificity
Clone Y12 is a mouse monoclonal antibody that detects Smith Antigen.
Evaluated by Western Blotting in HCT116 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Smith Antigen in HCT116 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:500 dilution from a representative lot detected Smith Antigen in Caco-2 cell lysate.
Immunocytochemistry Analysis: A representative lot detected Smith Antigen in Immunocytochemistry (dos Santos, N.R. et al. (1997). Hum Mol Genet. 6(9): 1549-58).
Western Blotting Analysis: A representative lot detected Smith Antigen in Western Blotting applications (Naro, C. et al. (2019). Cell Rep. 26(11): 2929-2941; Somarelli, J.A., et al. (2011). Lupus. 20(3): 274-89; Brahms, H. et al. (2000). J Biol Chem. 275(22): 17122-9).
RNA Binding Protein Immunoprecipitation (RIP): A representative lot detected Smith Antigen in RNA Binding Protein Immunoprecipitation applications (Naro, C. et al. (2019). Cell Rep. 26(11):2929-2941).
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Smith Antigen in human tonsil tissue sections.
ELISA Analysis: A representative lot detected Smith Antigen in ELISA applications (Zhou, Y, et al. (2017). J Immunol. 198(5):1846-1854).
Immunohistochemistry Applications: A representative lot detected Smith Antigen in Immunohistochemistry applications (Mirra, A., et al. (2017). Sci Rep. 7(1):2033).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Smith Antigen in Immunoprecipitation applications (Learner, E.A., et al. (1981). Proc Natl Acad Sci USA. 78(5):2737-41; Naro, C. et al. (2019). Cell Rep. 26(11): 2929-2941; Mirra, A., et al. (2017). Sci Rep.;7(1): 2033; Fischer, U. et al. (1997). Cell. 90(6):1023-9).
Radioimmunoassay: A representative lot detected Smith Antigen in Radioimmunoassay applications (Learner, E.A., et al. (1981). Proc Natl Acad Sci USA.;78(5):2737-41).
Immunofluorescence Analysis: A representative lot detected Smith Antigen in Immunofluorescence applications (Learner, E.A., et al. (1981). Proc Natl Acad Sci USA.78(5):2737-41; Mirra, A., et al. (2017). Sci Rep. 7(1):2033).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Smith Antigen, clone Y12, Cat. No. MABF2793, a mouse monoclonal antibody detects Smith antigen and is tested in ELISA, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, RIA, RNA Binding Protein IP (RIP), and Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Quality Level | 200 |
biological source | mouse |
antibody form | purified antibody |
antibody product type | primary antibodies |
clone | Y12, monoclonal |
mol wt | calculated mol wt 25 kDa","observed mol wt ~25 kDa |
purified by | using protein G |
species reactivity | mouse, human |
packaging | antibody small pack of 100 µL |
application(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable","immunoprecipitation (IP): suitable","western blot: suitable |
isotype | IgG3 |
conjugate | unconjugated |
UniProt accession no. | N/A, |
shipped in | 2-8°C |
WGK Germany | WGK 1 |
Flash Point F | Not applicable |
Flash Point C | Not applicable |
Получите коммерческое предложение в течение 1 часа
Менеджер подготовит коммерческое предложение и позвонит, если понадобится уточнить детали вашего заказа

С 2010 года мы поставляем оборудование с заводов Европы. Берем на себя все — от подбора оборудования до внедрения на предприятии

Все сотрудники имеют высшее образование, закончили ведущие химические вузы страны, такие как РХТУ им Менделеева.

У большинства компаний срок ожидания составляет 10-12 недель.

Оборудование хранится на сухом отапливаемом складе, где поддерживается ровная температура.

Работаем с PonyExpress и Деловыми линиями. Вы также можете выбрать свою транспортную компанию или забрать товар со склада в Москве.

В случае любых неполадок за свой счет выполним ремонт в сервисном центре или на заводе-изготовителе. Или бесплатно заменим прибор на новый.

Производим пуско-наладку оборудования, валидацию, обучение сотрудников. Если нужно, привлекаем инженеров с заводов- изготовителей.