Anti-Smad2/3 Antibody

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

7-408-25UL

7-408

7-408-100UL

SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. Like many other TGFβ family members SMAD2 is involved in cell signalling. SMAD2 modulates signals of activin and TGFbeta′s. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.
Specificity
Recognizes human Smad2 Mr 55-60 kDa and Smad3 Mr 50 kDa as determined by using Smad2 and Smad3 transfected Cos cells.
Fusion protein corresponding to amino acids 186-273 of human Smad2.
Research Sub Category
Transcription Factors
Immunoblot Analysis: 1:1000-1:2000 dilution detected Smad2/3 in RIPA lysates of HepG2 cells.
Optimal working dilutiuons must be determined by end user.
Research Category
Epigenetics & Nuclear Function
Anti-Smad2/3 Antibody is a Rabbit Polyclonal Antibody for detection of Smad2/3 also known as MAD-mothers against decapentaplegic-homolog 2 & has been validated in WB.
Quality
Routinely evaluated by Western Blot on Hela lysates.
Western Blot Analysis:
1:500 dilution of this lot detected SMAD2/3 on 10 µg of Hela lysates.
Target description
50-60 kDa
Replaces: 04-1029
Physical form
Purified rabbit polyclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.
ImmunoAffinity Purified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
HepG2 cell lysate.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	mouse, rat, human
packaging	antibody small pack of 25 µL
Торговая марка	Upstate®
application(s)	western blot: suitable
isotype	IgG
NCBI accession no.	NP_001003652,
UniProt accession no.	Q15796,
shipped in	ambient
Gene Information	human ... SMAD2(4087)