Anti-Sirt1(Sir2) Antibody, clone 3-10 Antibody, rabbit monoclonal

eCl@ss:

32160702

Кат. номер

MABE426-I-25UL

MABE426-I-100UL

NAD-dependent protein deacetylase sirtuin-1 (UniProt: Q923E4; also known as Regulatory protein SIR2 homolog 1, SIR2-like protein 1, SIR2alpha, Sir2, mSIR2a, Sirt-1) is encoded by the Sirt1 (also known as Sir2l1) gene (Gene ID: 93759) in murine species. Sirt1 is a NAD-dependent protein deacetylase that is widely expressed and links transcriptional regulation directly to intracellular energetics. In liver, Sirt1 is expressed in a circadian manner with maximal and minimal levels reaching at around Zeitgeber time (ZT) 16 and ZT4, respectively and its deacetylase activity is also regulated in a circadian manner that peaks at ZT15. It participates in the coordination of several cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis, and autophagy. It is shown to modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. It deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression in both positive and negative manner. Sirt1 serves as a sensor of the cytosolic ratio of NAD+/NADH, which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Sirt1 has four nucleotide (NAD)-binding regions and four zinc binding sites. It is reported to shuttle between nucleus and cytoplasm. Sir1 is proteolytically cleaved by cathepsin B upon TNF-alpha treatment to yield a catalytic inactive but stable 75 kDa fragment (aa 2-525). Sirt1 can be phosphorylated by multiple protein kinases. It is phosphorylated by STK4/MST1 that results in inhibition of SIRT1-mediated p53/TP53 deacetylation and phosphorylation by MAPK8/JNK1 at serine 46 and threonine 522 is reported to increase its nuclear localization and enzymatic activity. Sirt1 phosphorylation at threonine 522 by DYRK1A and DYRK3 activates its deacetylase activity and can promote cell survival.
Specificity
Clone 3-10 specifically detects murine Sirt1.
His-tagged full-length recombinant mouse NAD-dependent protein deacetylase sirtuin-1 (Sirt1).
Anti-Sirt1(Sir2), clone 3-10, Cat. No. MABE426-I, is a highly specific rabbit monoclonal antibody that targets NAD-dependent protein deacetylase sirtuin-1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Sirt1(Sir2) in mouse cerebral cortex tissue sections.
Quality
Evaluated by Western Blotting in NIH/3T3 cell lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Sirt1(Sir2) in NIH/3T3 cell lysate.
Target description
~110 kDa observed; 80.37 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Unpurified
Rabbit monoclonal antibody in cell culture supernatant with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
antibody form	unpurified
antibody product type	primary antibodies
clone	3-10, monoclonal
species reactivity	mouse
packaging	antibody small pack of 25 µL
application(s)	immunohistochemistry: suitable (paraffin)","western blot: suitable
isotype	IgG
NCBI accession no.	NP_062786,
UniProt accession no.	Q923E4,