Anti-SETD6

eCl@ss:

32160702

Кат. номер

ABE1337-25UG

ABE1337-100UG

N-lysine methyltransferase SETD6 (UniProt: Q8TBK2; also known as SET domain-containing protein 6) is encoded by the SETD6 gene (Gene ID: 79918) in human. N-lysine methyltransferase SETD6 is a SET domain protein that specifically monomethylates lysine 310 of NF-kappa B-p65 that leads to down-regulation of NF-kappa B transcription factor activity. It can also monomethylate lysine 8 of H2AZ (H2AZK8me1). SETD6 is required for the maintenance of embryonic stem cell self-renewal. Primary urothelial cells and normal bladder tissue have almost undetectable SETD6 levels that are shown to increase in transformed urothelial cells and is further increased in bladder cancer cells and tissues. Overexpression of SETD6 in transformed urothelial cells increases their survival and colony formation. SETD6 is shown to interact with the oxidative stress sensor protein DJ1, which is required for Nrf2-dependent transcription of antioxidant target genes. Under basal conditions, SETD6 and DJ1 are associated at chromatin and SETD6 inhibits DJ1 activity, which leads to the repression of Nrf2-dependent transcription. However, under conditions of oxidative stress SETD6 mRNA and protein levels decline that leads to elevation in Nrf2 expression and diminish interaction between SETD6 and DJ1. Two isoforms of SETD6 have been described that are produced by alternative splicing. (Ref.: Mukherjee, N., et al. (2017). Oncotarget. 8(9); 15114-15125; Chen, A., et al. (2016). Biochim. Biophys. Acta. 1859(2):420-427).
Specificity
This rabbit polyclonal antibody detects N-lysine methyltransferase SETD6 in human cells. it targets an epitope within 18 amino acids from the N-terminal region.
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human N-lysine methyltransferase SETD6, isoform 1.
Anti-SETD6, Cat. No. ABE1337, is a highly specific rabbit polyclonal antibody that targets N-lysine methyltransferase SETD6 and has been tested for use in Immunocytochemistry and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SETD6 in HEK293 cells.
Quality
Evaluated by Western Blotting in human kidney tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected SETD6 in human kidney tissue lysate.
Target description
~53 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	immunocytochemistry: suitable","western blot: suitable
NCBI accession no.	NP_001153777.1,
UniProt accession no.	Q8TBK2,

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable