Anti-SARS-CoV-2 spike protein ectodomain (S1+S2)

Кат. номер

ABF1066-25UL

ABF1066-100UL

Spike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 domain (aa 686-1213), which is reported to be well conserved, is responsible for membrane fusion. This polyclonal antibody binds and neutralizes the SARS-CoV-2 variants including the B.1.1.7; B.1.429; P.1; and B.1.351 at different levels. (Ref.: Ravichandran, S., et al. (2020). Sci Transl Med. 12(550):eabc3539).
Specificity
This rabbit polyclonal antibody detects the extracellular domain (S1+ S2) of SARS-CoV-2 spike protein.
His-tagged recombinant fragment corresponding to the extracellular domain (S1 + S2; aa 16-1213) of SARS-CoV-2 spike protein.
Anti-SARS-CoV-2 spike protein ectodomain (S1+S2), Cat. No. ABF1066, is a rabbit polyclonal antibody that detects the extracellular domain (S1+ S2) of SARS-CoV-2 spike protein and is tested for use in ELISA, Neutralizing, and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting with recombinant extracellular domain (S1+S2) of SARS-CoV-2 spike protein.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant extracellular domain (S1 + S2) of Spike glycoprotein of SARS-CoV-2.
Tested Applications
Enzyme Immunoassay (ELISA) Analysis: A representative lot detected SARS-CoV-2 spike protein ectodomain (S1+S2) in ELISA applications (Ravichandran, S., et. al. (2020). Sci Transl Med. 12(550):eabc3539).
Neutralizing: A representative lot neutralized SARS-CoV-2 spike protein ectodomain (S1+S2) in Neutralizing applications (Ravichandran, S., et. al. (2020). Sci Transl Med. 12(550):eabc3539).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Physical form
Rabbit polyclonal antibody in serum without preservatives.
Storage and Stability
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	purified antibody","serum
antibody product type	primary antibodies
clone	polyclonal
mol wt	calculated mol wt 139.13 kDa","observed mol wt ~170 kDa
purified by	(Unpurified)
species reactivity	virus
packaging	antibody small pack of 100 µL
application(s)	ELISA: suitable","neutralization: suitable","western blot: suitable
epitope sequence	Unknown
conjugate	unconjugated
Protein ID accession no.	YP_009724390,
UniProt accession no.	P0DTC2,
shipped in	dry ice
Gene Information	vaccinia virus ... S(43740568)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable