Anti-Rubicon antibody, Mouse monoclonal

Кат. номер

SAB4200838-25UL

SAB4200838-100UL

Run domain Beclin-1-interacting and cysteine-rich domain-containing protein (Rubicon) also known as Beclin-1 associated RUN domain containing protein (Baron) or KIAA0226, is a negative regulator of endosomes maturation and endocytic trafficking.1-2 Rubicon knockdown studies showed enhanced autophagosome maturation and endocytosis.1 It also have a critical role in the LC3-associated phagocytosis (LAP) a noncanonical autophagy process which can be induced through the activation of extracellular receptor by different stimulators including pathogens, immune complexes and dying cells.4-5
Specificity
Monoclonal Anti-Rubicon antibody specifically recognizes Rubicon from human origin.
synthetic peptide corresponding to the N-terminal region of human Rubicon, conjugated to KLH
The antibody may be used in various immunochemical techniques including Immunoblotting and Immunofluorescence. Detection of the Rubicon band by Immunoblotting is specifically inhibited by the immunogen.
Biochem/physiol Actions
The mammalian class III phosphatidylinositol 3-kinase (PtdIns3K) complex consists of three core proteins, catalytic VPS34, the adaptor VPS15 (p150) and the recruiter Beclin-1 (ATG6); these endocytosis regulators were reported to stably bind Beclin-1 ATG14, UVRAG and Rubicon.1-3 The complex Rubicon-UVRAG–Beclin-1/PtdIns3K can be found both in early and in late endosomes or lysosomes. Rubicon was described as the mediator for the recruitment of the UVRAG-Beclin-1-VPS34 complex and of ATG7 and LC3-II LAPosome.4-5 Furthermore, Rubicon has been identified as a key modulator of the inflammatory response and viral replication, during several viral infections such as hepatitis B virus (HBV). Rubicon expression is induced in negative regulation of the innate immune response resulting in enhances viral replication and possibly supporting viral immune evasion mechanism.4
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified from hybridoma cell culture
antibody product type	primary antibodies
clone	RB-145, monoclonal
form	liquid
species reactivity	human
packaging	antibody small pack of 25 µL
concentration	~1 mg/mL
application(s)	immunoblotting: 2-4 µg/mL using human A431 whole cell extract.","immunofluorescence: 2-4 µg/mL using human HeLa cells.
isotype	IgG1
UniProt accession no.	Q92622,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... RUBCN(9711)

Flash Point F	Not applicable
Flash Point C	Not applicable