Anti-RTN4IP1 Antibody, clone NIMPR14 MABC961-25UL

eCl@ss:

32160702

Reticulon-4-interacting protein 1, mitochondrial (UniProt: Q924D0; also known as NOGO-interacting mitochondrial protein, RTN4IP1) is encoded by the Rtn4ip1 (also known as Nimp) gene in murine species. RTN4IP1 is an outer mitochondrial membrane protein that interacts with reticulon 4, which is a potent inhibitor of regeneration following spinal cord injury. This interaction may be important for reticulon-induced inhibition of neurite growth. RNT4IP1 plays a role in the regulation of retinal ganglion cell (RGC) neurite outgrowth, and hence in the development of the inner retina and optic nerve. It is widely expressed in mitochondria-enriched tissues and co-localizes with the endoplasmic reticulum HSPA5 at spots corresponding to contacts with mitochondria. Mutations in Rtn4ip1 gene can cause optic atrophy 10 that leads to vision loss sometime in early childhood.
Specificity
Clone NIMPR14 is a rat monoclonal antibody that detects Reticulon-4-interacting protein 1 in murine species.
Purified BALB/c mouse neutrophils.
Flow Cytometry Analysis: A representative lot detected RTN4IP1 in Flow Cytometry applications (Lopez, A.F., et. al. (1984). Br J Haematol. 57(3):489-94; Taylor, P.R., et. al. (2014). Nat Immunol. 15(2):143-51).
Inhibition Analysis: A representative lot detected RTN4IP1 in Inhibition applications (Tacchini-Cottier, F., et. al. (2000). J Immunol. 165(5):2628-36; de Vries, B., et. al. (2003). J Immunol. 170(7):3883-9; Chang, H.R., et. al. (1993). J Leukoc Biol. 53(6):636-9; Mentzel, S., et. al. (1999). Kidney Int. 55(4):1335-47).
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected RTN4IP1 in mouse lung tissue.
Immunohistochemistry Analysis: A representative lot detected RTN4IP1 in Immunohistochemistry applications (Lopez, A.F., et. al. (1984). Br J Haematol. 57(3):489-94).
Immunohistochemistry Analysis: A representative lot detected RTN4IP1 in Immunohistochemistry applications (Lopez, A.F., et. al. (1984). Br J Haematol. 57(3):489-94.; de Vries, B., et. al. (2003). J Immunol. 170(7):3883-9; Chang, H.R., et. al. (1993). J Leukoc Biol. 53(6):636-9).
Immunocytochemistry Analysis: A representative lot detected RTN4IP1 in Immunocytochemistry applications (Lopez, A.F., et. al. (1984). Br J Haematol. 57(3):489-94.; Taylor, P.R., et. al. (2014). Nat Immunol. 15(2):143-51).
Detect Reticulon-4-interacting protein 1, mitochondrial using this rat monoclonal Anti-RTN4IP1, clone NIMP‐R14 , Cat. No. MABC961-25UL, validated for use in Immunohistochemistry, Inhibition and Immunohistochemistry (Paraffin).
Research Category
Apoptosis & Cancer
Quality
Evaluated by Immunohistochemistry in mouse spleen tissue.
Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected RTN4IP1 in mouse spleen tissue.
Physical form
Format: Purified
Purified rat monoclonal antibody IgG2b in PBS without preservatives.
Protein G purified
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	rat
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	monoclonal
species reactivity	mouse
packaging	antibody small pack of 25 µL
application(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable","inhibition assay: suitable
isotype	IgG2b
UniProt accession no.	Q924D0,

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable