Anti-RPA2 p34 Antibody, clone RPA20 1-46

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

4-1481-25UG

4-1481

4-1481-100UG

RPA is a heterotrimeric protein complex that binds specifically to single-stranded DNA (ssDNA). It is composed of three subunits, RPA1 (70 kDa), RPA2 (32 kDa), and RPA3 (14 kDa), and plays multiple roles in DNA metabolism. RPA is required for DNA replication initiation, as well as replication elongation. At the onset of DNA replication, RPA is loaded onto chromatin after the binding of Cdc45 to origins. RPA is needed for subsequent loading of DNA polymerase and other replication proteins to initiate DNA replication. After DNA replication begins, RPA moves with replication forks, stabilizing ssDNA and assisting in DNA synthesis. In addition to its replication function, RPA is also known to play essential roles in damage repair and recombination.The 32 kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK. Alternate names for RPA32 include replication protein A 32 kDa subunit, RP-A, RF-A, replication factor-A protein 2, p32, p34, RPA2, REPA2, and RPA32.
Specificity
This antibody recognizes RPA2.
His-tagged recombinant protein corresponding to human RPA2.
Epitope: Unknown
Research Sub Category
Cell Cycle, DNA Replication & Repair
Chromatin Biology
Anti-RPA2 p34 Antibody, clone RPA20 1-46 is a Mouse Monoclonal Antibody for detection of RPA2 p34 also known as Replication factor A protein 2 & has been validated in WB.
Western Blot (SNAP ID) Analysis: 0.5 µg/mL from a previous lot detected RPA2 on 10 µg of NIH/3T3 cell lysate.
Research Category
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected RPA2 on 10 µg of HeLa cell lysate.
Target description
~32 kDa
Physical form
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	1-46, monoclonal","RPA20, monoclonal
species reactivity	mouse, human
packaging	antibody small pack of 25 µg
application(s)	western blot: suitable
isotype	IgG1
NCBI accession no.	NM_002946,
UniProt accession no.	P15927,
shipped in	ambient