Anti-RNA polymerase II subunit B1 Antibody, clone 4F8

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABE30-25UG

ABE30

RNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II complex. As a holoenzyme RNA polymerase II catalyzes transcription of eukaryotic DNA into RNA using the four ribonucleoside triphosphates as substrates. The RPB1 subunit, in combination with other polymerase subunits, forms a large central cleft that maintains contact between the active site of the enzyme, the DNA template, and the nascent RNA transcript. This subunit also contains a carboxy terminal domain (CTD) consisting of tandem heptapeptide repeats. In actively transcribing RNA polymerase ‘Ser-2’ and ‘Ser-5’ of the heptapeptide repeat are phosphorylated. Phosphorylation activates the RNA polymerase II beta subunit, allowing it to serve as an assembly platform for additional subunits that modulate initiation, elongation, termination and mRNA processing.
KLH-conjugated linear peptide corresponding to human RNA polymerase II subunit B1.
Use Anti-RNA polymerase II subunit B1 Antibody, clone 4F8 (rabbit polyclonal antibody) validated in WB, ICC to detect RNA polymerase II subunit B1 also known as DNA-directed RNA polymerase II subunit RPB1.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected RNA polymerase II subunit B1 in HeLa cells.
Quality
Evaluated by Western Blot in HeLa nuclear extract.
Western Blot Analysis: 1 µg/mL of this antibody detected RNA polymerase II subunit B1 on 10 µg of HeLa nuclear extract.
Target description
~217 kDa observed
Physical form
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa nuclear extract
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	human
species reactivity (predicted by homology)	mouse (based on 100% sequence homology), horse (based on 100% sequence homology), bovine (based on 100% sequence homology), canine (based on 100% sequence homology), rat (based on 100% sequence homology)
packaging	antibody small pack of 25 µg
application(s)	immunocytochemistry: suitable","western blot: suitable
NCBI accession no.	NP_000928,
UniProt accession no.	P24928,
shipped in	wet ice

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable