Anti-RNA polymerase II Antibody, clone CTD4H8

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

5-623

5-623-25UG

5-623-100UG

RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
Specificity
This antibody recognizes Phospho- and non-phospho-RNA polymerase II.
Peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.
Research Sub Category
Transcription Factors
Anti-RNA polymerase II Antibody, clone CTD4H8 is a high quality Mouse Monoclonal Antibody for the detection of RNA polymerase II and has been published in more than 40 citations and validated for use in ChIP & WB.
Research Category
Epigenetics & Nuclear Function
Quality
Routinely evaluated by western blot on HeLa nuclear extracts.
Western Blot Analysis:
0.05-1 µg/mL of this lot detected RNA polymerase II in HeLa nuclear extracts. 0.1-2 µg/mL of a previous lot detected RNA polymerase II in 3T3 nuclear extracts
Target description
210-220 kDa
Physical form
Format: Purified
Protein G Chromatography
Purified mouse IgG1 in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
A431 nuclear extract.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	CTD4H8, monoclonal
species reactivity	mouse, Saccharomyces cerevisiae, rat, human
packaging	antibody small pack of 25 µg
Торговая марка	Upstate®
application(s)	ChIP: suitable (ChIP-seq)","western blot: suitable
isotype	IgG1
NCBI accession no.	NP_000928,
UniProt accession no.	P24928,
shipped in	dry ice