Anti-RKIP Antibody, clone 4A11

eCl@ss:

32160702

Кат. номер

MABC1137-100UG

MABC1137-25UG

Phosphatidylethanolamine-binding protein 1 (UniProt: P30086; also known as PEBP-1, HCNPpp, Neuropolypeptide h3, Prostatic-binding protein, Raf kinase inhibitor protein, RKIP) is encoded by the PEBP1 (also known as PBP, PEBP) gene (Gene ID: 5037) in human. RKIP is as member of the phosphatidylethanolamine-binding-protein (PEBP) family that antagonizes multiple cell-survival pathways, such as the Ras-Raf-1, MEK/ERK, NF- B, and G-protein-coupled receptor Kinase 2 and thereby modulates cell growth, apoptosis, motility, invasion and metastasis. Over-expression of RKIP is shown to inhibit c-Src auto-phosphorylation and activation and IL-6-, JAK1 and 2-, and Raf-mediated STAT3 phosphorylation and activation. RKIP overexpression is shown to sensitize tumor cells to chemotherapeutic drug-induced apoptosis. Hence, it is considered as a suppressor of metastasis and a prognostic marker in several cancers. Clone 4A11 exhibits specific staining in formalin-fixed NIH3T3 cells transfected with GFP-PEBP1 and in some pancreatic cancer cells. (Ref.: Odabaei, G., et al. (2004). Adv. Cancer Res. 91; 169-200; Yousuf, S., et al. (2014). PLoS One 9(3); e92478).
Specificity
Clone 4A11 specifically detects human Raf kinase inhibitor protein, (RKIP).
Purified native and denatured Raf kinase inhibitor protein (RKIP).
Detects Raf kinase inhibitor protein (Phosphatidylethanolamine-binding protein 1) using this mouse monoclonal Anti-RKIP, clone 4A11, Cat. No. MABC1137, tested for use in Western Blotting, Immunocytochemistry, and Immunohistochemistry (Paraffin).
Research Category
Apoptosis & Cancer
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected RKIP in human pancreas and human thyroid tissue sections.
Immunohistochemistry Analysis: A representative lot of this antibody detected RKIP in Immunohistochemistry applications (Wang, X., et. al. (2010). J Immunol Methods. 362(1-2):151-60).
Immunocytochemistry Analysis: A representative lot of this antibody detected RKIP in Immunocytochemistry applications (Wang, X., et. al. (2010). J Immunol Methods. 362(1-2):151-60).
Quality
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected RKIP in HEK293 cell lysate.
Target description
~23 kDa; 21.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Purified mouse monoclonal antibodyIgG1κ in buffer containing PBS without azide
Protein G purified
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	4A11, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	immunocytochemistry: suitable","immunohistochemistry: suitable (paraffin)","western blot: suitable
isotype	IgG1
NCBI accession no.	NP_002558.1,
UniProt accession no.	P30086,
Gene Information	human ... PEBP1(5037)