Anti-Reptin antibody, Mouse monoclonal

NACRES:

NA.41

Кат. номер

SAB4200115-25UL

SAB4200115-200UL

Monoclonal Anti-Reptin (mouse IgG1 isotype) is derived from the hybridoma 2E9-5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a human Reptin fusion protein.
Reptin, also known as RuvB-like 2 RUVBL2, is encoded by the gene mapped to human chromosome 19q13.33. The encoded protein belongs to the AAA+ family of DNA helicases.
a human Reptin fusion protein. The corresponding sequence differs by one amino acid in mouse.
Monoclonal Anti-Reptin antibody produced in mouse has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence
• confocal microscopy

Biochem/physiol Actions
Reptin and pontin are associated with several chromatin-remodeling complexes and are involved in multiple biological processes including chromatin remodeling, DNA damage repair, telomerase activity, transcriptional regulation, apoptosis and cancer metastasis. Both proteins are also involved in cellular transformation by β-catenin and c-myc through their chromatin remodeling function.
Reptin has been involved in various cellular processes, including the response to DNA double-strand breaks and the control of gene expression. The encoded protein plays a vital role in repression of specific β-catenin and nuclear factor-κB targets. Elevated expression of the gene leads to the development of hepatocellular carcinomas (HCC) and renal cell carcinoma (RCC). Thus, reptin can be considered as a potential therapeutic target for HCC and RCC.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified from hybridoma cell culture
antibody product type	primary antibodies
clone	2E9-5, monoclonal
form	buffered aqueous solution
mol wt	antigen ~51 kDa
species reactivity	mouse, human, rat
packaging	antibody small pack of 25 µL
concentration	~1.0 mg/mL
application(s)	western blot: 0.5-1.0 µg/mL using whole extract of human MCF-7 cells
conjugate	unconjugated
UniProt accession no.	Q9Y230,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... RUVBL2(10856)

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable