Anti-RAMP1 Antibody, clone 4F8.1

eCl@ss:

32160702

Кат. номер

MABS1904-25UG

MABS1904-100UG

Receptor activity-modifying protein 1 (UniProt: O60894; also known as Calcitonin-receptor-like receptor activity-modifying protein 1, CRLR activity-modifying protein 1, RAMP1) is encoded by the RAMP1 gene (Gene ID: 10267) in human. RAMP1 is a single-pass type I membrane protein that is involved in the transports of calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane and acts as a receptor for calcitonin-gene-related peptide (CGRP) together with CALCRL. RAMP1 lacks glycosylation sites and does not migrate to the cell surface independently. To reach the cell surface, it aggregates inside the cytoplasm and forms heterodimers with calcitonin. It is expressed in many tissues including the uterus, bladder, brain, pancreas, retina, and gastro-intestinal tract. It is synthesized with a signal peptide (aa 1-26), which is cleaved to generate the mature form. Mature RAMP1 has an extracellular domain (aa 27-117); a transmembrane domain (aa 118-138); and a short cytoplasmic tail (aa 139-148). RAMP1 is reported to acts as a vasodilation factor and mice deficient in RAMP1 exhibit inflammatory responses with a transient, but significant, increase in serum calcitonin-gene-related peptide levels and pro-inflammatory cytokines. (Ref.: Nakayama, T., et al. (2017). J. Hum. Hypertens. 31(5); 361-365).
Specificity
Clone 4F8.1 specifically detects Receptor activity-modifying protein 1 (RAMP1) in human cells. It targets an epitope within 91 amino acids from the internal region.
GST/His-tagged recombinant fragment corresponding to 91 amino acids from the internal region of human Receptor activity-modifying protein 1 (RAMP1).
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected RAMP1 in human uterus and human colon tissue sections.
Research Category
Signaling
Anti-RAMP1, clone 4F8.1, Cat. No. MABS1904, is a mouse monoclonal antibody that detects Receptor activity-modifying protein 1 (RAMP1) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Quality
Evaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected RAMP1 in human brain tissue lysate.
Target description
~17 kDa observed; 16.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	4F8.1, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	immunohistochemistry: suitable (paraffin)","western blot: suitable
isotype	IgG2b
NCBI accession no.	NP_005846,
UniProt accession no.	O60894,

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable