Anti-Rae1 Antibody, clone 14G7.1

eCl@ss:

32160702

Кат. номер

MABC1233-25UL

MABC1233-100UL

mRNA export factor (UniProt: P78406; also known as Rae1 protein homolog, mRNA-associated protein mrnp 41) is encoded by the RAE1 (also known as MRNP41) gene (Gene ID: 8480) in human. mRNA export factor (UniProt: P78406; also known as RAE1 protein homolog, mRNA-associated protein mrnp 41) is encoded by the RAE1 (also known as MRNP41) gene (Gene ID: 8480) in human. Rae1 is a member of the superfamily of WD-repeat proteins that plays a role in mitotic bipolar spindle formation. It contains seven WD repeats. Rae1 is also reported to bind to a GLEBS-like NUP98 motif at the nuclear pore complex through multiple domains, including WD-repeats. Also, the COOH-Terminal non- WD-repeat extension of Rae1 Is shown to be essential for NUP98 binding. Rae1 plays a role in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. It is recruited from interphase nuclei to spindle microtubules during mitosis. RAE1 also interacts with NUMA1 via N-terminal end of the coiled-coil domain and this interaction promotes spindle formation in mitosis. (Ref.: Pritchard, CEJ et al. (1999). J. cell Biol. 145(2); 237-254).
Specificity
Clone 14G7.1 specifically detects human mRNA export factor (Rae1). it targets an epitope with in the C-terminal half.
GST/His-tagged recombinant fragment corresponding to 179 amino acids from the C-terminal half of human mRNA export factor (Rae1).
Anti-Rae1, clone 14G7.1, Cat. No. MABC1233, is a mouse monoclonal antibody that detects mRNA export factor (Rae1) and has been tested for use in Western Blotting.
Research Category
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rae1 in HEK293 cell lysate.
Target description
~40 kDa observed; 40.97 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	14G7.1, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µL
application(s)	western blot: suitable
isotype	IgG2b
NCBI accession no.	NP_001015885,
UniProt accession no.	P78406,

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable