- Производитель:
- Sigma-Aldrich
NACRES: | NA.41 |
Кат. номер |
SAB4200174-25UL |
SAB4200174-200UL |
Specificity
Anti-Rab18 (C-terminal) recognizes human, mouse, and rat Rab18.
- immunoblotting
- immunoprecipitation
- immunofluorescence
Biochem/physiol Actions
Ras-associated binding (Rab)-guanosine triphosphatases (GTPases) are central regulators of membrane trafficking between different subcellular compartments of eukaryotic cells. Their regulatory capacity depends on their ability to cycle between the guanosine diphosphate (GDP)-bound inactive and guanosine triphosphate (GTP)-bound active states. Rab18 is involved in retrograde Golgi-endoplasmic reticulum (ER) trafficking. It localizes to lipid droplets and induces the association of the lipid droplets with endoplasmic reticulum (ER) membranes. Anti-Rab18 may thus be used as a marker to follow the dynamics of lipid droplets in living cells. Rab18 has a potential function in eye development. Mutations in the RAB18 are implicated in Warburg micro syndrome.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Storage and Stability
Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Discard working dilutions if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source | rabbit |
antibody form | affinity isolated antibody |
antibody product type | primary antibodies |
clone | polyclonal |
form | buffered aqueous solution |
mol wt | ~23 kDa (additional bands of higher MW may appear in some extract preparations) |
species reactivity | mouse, rat, human |
packaging | antibody small pack of 25 µL |
concentration | ~1.0 mg/mL |
application(s) | immunoprecipitation (IP): 2.5-5.0 µg using lysates of human PC3 cells","indirect immunofluorescence: 2.5-5.0 µg/mL using rat NRK cells","western blot: 2.5-5.0 µg/mL using whole extracts of mouse MM3MG cells |
conjugate | unconjugated |
UniProt accession no. | Q9NP72, |
shipped in | dry ice |
storage temp. | 20°C |
Gene Information | human ... RAB18(22931) mouse ... Rab18(19330) rat ... Rab18(307039) |
RIDADR | NONH for all modes of transport |
Flash Point F | Not applicable |
Flash Point C | Not applicable |
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