Anti-Profilin 2 (C-terminal) antibody produced in rabbit

NACRES:

NA.41

Кат. номер

P0101-25UL

P0101-200UL

Profilins are actin-monomer-binding proteins, abundantly expressed in all eukaryotic cells from yeast to mammals. Profilin 2 (PFN2) is the neuronal specific isoform. It is an actin-binding protein and is a member of the small G-actin-binding class of protein.
synthetic peptide corresponding to amion acid residues 128-140 of human profilin 2 conjugated to KLH. The corresponding sequence is identical in mouse and rat.
Anti-Profilin 2 (C-terminal) antibody produced in rabbit has been used in immunostaining and immunoblotting.
Biochem/physiol Actions
Profilins are key regulators of actin dynamics. Profilin 2 (PFN2) associates with a number of proteins involved in membrane trafficking and endocytosis, such as synapsin and dynamin 1. It associates with dynamin 1 through the C-terminal proline-rich domain of dynamin 1 and thereby competes with the binding of SH3 ligands, such as endophilin and amphiphysin, thus interfering with the assembly of the endocytic machinery. Overexpression of profilin 2 inhibits endocytosis. Absence of profilin 2 in neurons increases endocytosis and membrane recycling. Profilin 2 is required for stimulated actin polymerization in the synapse and is involved in the regulation of synaptic vesicle exocytosis, neuronal excitability and complex behavior.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	IgG fraction of antiserum
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~14 kDa
species reactivity	human, mouse, rat
packaging	antibody small pack of 25 µL
application(s)	western blot: 1:500-1:1,000 using whole extracts of human SH-SY5Y cells, mouse brain, and rat brain
conjugate	unconjugated
UniProt accession no.	P35080,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... PFN2(5217) mouse ... Pfn2(18645) rat ... Pfn2(81531)

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable