Anti-PRDM9

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABE1947

ABE1947-25UG

Histone-lysine N-methyltransferase PRDM9 (UniProt: Q9NQV7; EC: 2.1.1.43, PR domain zinc finger protein 9, PR domain-containing protein 9, PRDM9) is encoded by PRDM9 (also known as PFM6) gene (Gene ID: 56979) in human. PRDM9 is a zinc-finger protein that catalyzes histone H3 Lysine 4 trimethylation (H3K4me3), but lacks capacity to mono- and dimethylate Lysine 4 of histone H3. H3 Lysine -4 methylation plays a vital role in the transcriptional activation of genes during early meiotic prophase. PRDM9 contains multiple domains, including a Kruppel-associated box (KRAB) domain (aa 23-86), an SSX repression domain (SSXRD), a PRD1-BF1 and RIZ homologous region, a subclass of SET (PR/SET) domain (aa 244-358), and a tandem array of C2H2 zinc fingers. The zinc finger array recognizes a short sequence motif, leading to local H3K4me3, and meiotic recombination hotspot activity. PRDM9 is reported to be highly polymorphic, both within and between mammalian species. It is shown that most of the naturally occurring sequence polymorphisms in PRDM9 change the identity of the amino acids contacting DNA and/or the number and arrangement of individual fingers in the DNA-binding zinc-finger domains. This allows PRDM9 variants to target a large number of DNA sequences, thereby expanding the distribution of recombination sites. (Ref.: Baker, CL et al. (2015). PLoS Genet 11(9): e1005512).
Specificity
This rabbit polyclonal antibody detects Histone-lysine N-methyltransferase PRDM9 in human and mouse cells. It targets an epitope within 191 amino acids from the N-terminal half.
GST-tagged recombinant fragment corresponding to 191 amino acids from the N-terminal half of human Histone-lysine N-methyltransferase PRDM9.
Western Blotting Analysis: A representaive lot detected PRDM9 in Wild type, but not in KO mouse testis tissue lysate ( Courtesy of Guccione Lab @ A*STAR( Institute of Molecular Cell Biology, IMCB) ).
Anti-PRDM9, Cat. No. ABE1947, is a highly specific rabbit polyclonal antibody that targets Histone-lysine N-methyltransferase PRDM9 in human and murine cells and has been tested for use in Western Blotting.
Western Blotting Analysis: A representaive lot detected PRDM9 in Wild type, but not in KO mouse testis tissue lysate (Courtesy of Guccione Lab @ A*STAR(Institute of Molecular Cell Biology, IMCB)).
Research Category
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blotting in mouse testis tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected PRDM9 in 10 µg of mouse testis tissue lysate.
Target description
~90 kda observed; 103.37 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
species reactivity	human, mouse
packaging	antibody small pack of 25 µg
application(s)	western blot: suitable
isotype	IgG
NCBI accession no.	NP_064612,
UniProt accession no.	Q9NQV7,
shipped in	ambient

Flash Point F	does not flash
Flash Point C	does not flash