Anti-PPOX

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABS2123

ABS2123-25UL

Protoporphyrinogen oxidase (UniProt: P50336; also known as EC: 1.3.3.4, PPO, PPOX) is encoded by the PPOX gene (Gene ID: 5498) in human. Protoporphyrinogen oxidase is a mitochondrial inner membrane enzyme that catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX. It is involved in step one of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX. It is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas and may be present as a monomer or active homodimeric form. Defects in PPOX are linked to variegate porphyria that lead to severe deficiency of this enzyme that result in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver.
Specificity
This rabbit polyclonal antibody detects Protoporphyrinogen oxidase (PPO) in Human and Mouse cells.
Full length recombinant human Protoporphyrinogen oxidase (PPOX).
Epitope: unknown
Western Blotting Analysis: A representative lot detected PPOX in undifferentiated (day 0) and maturing (day 3 or 4) mouse erythroleukemia (MEL) cells (Chung, J., et. al. (2015). Sci Signal. 8(372):ra34). and differentiated Mouse erythroleukemia (MEL) cells (Medlock, A.E., et. al. (2015). PLoS One. 10(8):e0135896).
Anti-PPOX, Cat. No. ABS2123, is a rabbit polyclonal antibody that detects Protoporphyrinogen oxidase and has been tested for use in Western Blotting.
Research Category
Signaling
Quality
Evaluated by Western Blotting in lystate from mitochondria isolated from differentiated DS19 cells.
Western Blotting Analysis: A 1:500 dilution of this antibody detected PPOX in lystate from mitochondria isolated from differentiated DS19 cells.
Target description
~53 kDa observed; 50.77 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Unpurified
Unpurified
Rabbit polyclonal antiserum with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	unpurified
antibody product type	primary antibodies
clone	polyclonal
species reactivity	mouse
species reactivity (predicted by homology)	human (based on 100% sequence homology)
packaging	antibody small pack of 25 µL
application(s)	western blot: suitable
isotype	IgG
NCBI accession no.	NP_000300,
UniProt accession no.	P50336,
shipped in	ambient
Gene Information	human ... PPOX(5498)

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