Anti-POT1 Antibody, clone 43B7

Кат. номер

MABE1907-25UG

MABE1907-100UG

Protection of telomeres protein 1 (UniProt: Q9NUX5; also known as hPot1, POT1-like telomere end-binding protein) is encoded by the POT1 gene (Gene ID: 25913) in human. POT1 is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. As a component of the shelterin complex, it is involved in the regulation of telomere length and protection. It also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. It binds to two or more telomeric single-stranded 5′-TTAGGG-3′ repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5′-TAGGGTTAG-3′ sequence. Its DNA binding domains are localized to amino acids 33-48 and 270-273. POT1 contains two copies of oligonucleotide oligosaccharide-binding fold (OB-fold) domains in its N-terminal half that are responsible for single strand DNA binding activity. TPP1, a shelterin component, plays critical role in chromosome end protection and telomere length regulation and POT1 is shown to negatively regulate TPP1 phosphorylation at serine Ser255 by NIMA Related Kinase 6 (NEK6). (Ref.: Hirai, Y., et al. (2016). Genes Cells 21(8); 874-889; Hirai, Y., et al. (2012). Genes Cells. 17(3); 186-204).
Specificity
Clone 43B7 is a mouse monoclonal antibody that detects human Protection of telomeres protein 1 (POT1).
Full-length recombinant human Protection of telomeres protein 1 (POT1).
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Protection of telomeres protein 1 (POT1) in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected POT1 in HEK293T, HepG2, and K562 cell lysates.
Immunofluorescence Analysis: A representative lot detected POT1 in Immunofluorescence application. (Hirai, Y., et al. (2012). Genes Cells. 17(3):186-204).
Immunoprecipitation Analysis: A representative lot immunoprecipitated POT1 in Immunoprecipitation application. (Hirai, Y., et al. (2012). Genes Cells. 17(3):186-204; Hirai, Y. et al. (2016). Genes Cells. (8): 874-889).
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected POT1 in Chromatin Immunoprecipitation applications (Hirai, Y., et al. (2012). Genes Cells. 17(3):186-204).
Western Blotting using knockout lysates: A representative lot detected POT1 in wild-type, but not in KO lysates in Western Blotting application. (Hirai, Y., et al. (2012). Genes Cells. 17(3):186-204).
Western Blotting Analysis: A representative lot detected POT1 in Western Blotting application. (Hirai, Y. et al. (2016). Genes Cells. (8): 874-889).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-POT1, clone 43B7, Cat. No. MABE1907, is a mouse monoclonal antibody that detects POT1 and is tested for use in Chromatin Immunoprecipitation, Immunofluorescence, Immunoprecipitation, Western Blotting, and Western Blotting using KO lysates.
Physical form
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	43B7, monoclonal
mol wt	calculated mol wt 71.44 kDa","observed mol wt ~70 kDa
purified by	using protein G
species reactivity	human
packaging	antibody small pack of 100 µg
application(s)	immunoprecipitation (IP): suitable","western blot: suitable
isotype	IgG2a
epitope sequence	N-terminal
conjugate	unconjugated
Protein ID accession no.	NP_001036059,
UniProt accession no.	Q9NUX5,
shipped in	2-8°C
Gene Information	human ... POT1(25913)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable