Anti-Plakophilin 1 Antibody, clone 10B2

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

MABT1505-25UG

MABT1505-100UG

Plakophilin-1 (UniProt: Q13835; also known as Band 6 protein, B6P) is encoded by the PKP1 gene (Gene ID: 5317) in human. Plakophilins are proteins that are involved in linking adherins to cytoskeletal intermediate filaments. They contain numerous armadillo (ARM) repeats and localize to cell desmosomes and nuclei. Plakophilin-1 may be involved in molecular recruitment and stabilization during desmosome formation. It is also shown to be important for tooth development and regulates ZO-1 distribution in the dental epithelium. Two isoforms of Plankophilin-1 have been reported that are produced by alternative splicing. Isoform 1 is expressed in stratified squamous, complex, glandular duct and bladder epithelia and isoform 2 is widely expressed. Plakophilin-1 is shown to be highly expressed in teeth and skin, and was upregulated during tooth development. It is reported to be involved in the regulation of Wnt signaling via its ARM repeat domains in a manner similar to beta-catenin and Wnt activation induces its nuclear translocation. Mutations n PKP1 gene are shown to cause ectodermal dysplasia-skin fragility syndrome that is characterized by cutaneous fragility and congenital ectodermal dysplasia affecting skin, hair and nails. (Ref.: Sprecher, E., et al (2004). J. Invest. Dermatol. 122(3); 647-51; Miyazaki, K et al. (2016). PLoS ONE 11(3): e0152206).
Specificity
Clone 10B2 specifically detects Planophilin-1 and targets an epitope with in the first 27 amino acids from the N-terminal region.
MBP-conjugated recombinant fragment corresponding to the first 235 amino acids from the N-terminal region of human Plakophilin-1.
Epitope: N-terminus
Research Category
Cell Structure
Anti-Plakophilin 1, clone 10B2, Cat. No. MABT1505, is a mouse monoclonal antibody that detects Plantophilin 1 and has been tested for use in Immunofluorescence and Western Blotting.
Western Blotting Analysis: A representative lot detected Plakophilin 1 in Western Blotting applications (Pigors, M., et. al. (2011). Hum Mol Genet. 20(9):1811-9).
Immunofluorescence Analysis: A representative lot detected Plakophilin 1 in Immunofluorescence applications (Sprecher, E., et. al. (2004). J Invest Dermatol. 122(3):647-51;Pigors, M., et. al. (2011). Hum Mol Genet. 20(9):1811-9).
Quality
Evaluated by Western Blotting in PC3 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Plakophilin 1 in 10 µg of PC3 cell lysate.
Target description
~80 kDa observed; 80.50 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	10B2, monoclonal
species reactivity	human
species reactivity (predicted by homology)	rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
packaging	antibody small pack of 25 µg
application(s)	immunofluorescence: suitable","western blot: suitable
isotype	IgG1
NCBI accession no.	NP_000290,
UniProt accession no.	Q13835,
Gene Information	human ... PKP1(5317)