Anti-PI3 Kinase Antibody, p85 Antibody, N-SH3 Antibody, clone 1D5.1

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

MABS831

MABS831-25UG

Phosphatidylinositol 3-kinase regulatory subunit alpha (UniProt: P27986; also known as Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha) is encoded by the PIK3R1 (also known as GRB1) gene (Gene ID: 5295) in human. PI3K regulatory subunit alpha catalyzes the phosphorylation of PI(4,5)P2 inositol ring at the 3 position to generate PI(3,4,5)P3, a potent second messenger that mediates survival signaling and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Following ligand binding, tyrosine phosphorylation of growth factor receptors creates docking sites for p85 binding (through its SH2 domains), and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. PI3 kinase alpha plays a role in insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Mutations in PIK3R1 gene are known to be associated with insulin resistance.
Specificity
Clone 1D5.1 detects p85 subunit of human and murine PI-3 kinase alpha. It targets an epitope within 77 amino acids from the N-terminal region.
GST/His-tagged recombinant fragment corresponding to 77 amino acids from the N-terminal region of the p85 regulatory subunit of human PI 3-kinase alpha.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected PI3 Kinase, p85, N-SH3 in 10 µg of human cerebral cortex tissue lysate.
Anti-PI3 Kinase, p85, N-SH3, clone 1D5.1, Cat. No. MABS831, is a mouse monoclonal antibody that detects human and murine p85 subunit of PI3 kinase. It has been tested for use in Western Blotting.
Research Category
Signaling
Quality
Evaluated by Western Blotting in NIH/3T3 L1 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PI3 Kinase, p85, N-SH3 in 10 µg of NIH/3T3 L1 cell lysate.
Target description
~85 kDa observed; 83.60 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	1D5.1, monoclonal
species reactivity	human, mouse
packaging	antibody small pack of 25 µg
application(s)	western blot: suitable
isotype	IgG2b
NCBI accession no.	NP_852664,
UniProt accession no.	P27986,
shipped in	ambient
Gene Information	human ... PIK3R1(5295)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable