Anti-Phospholipase A2 (cPLA2) (N-terminal) antibody produced in rabbit

MDL number:	MFCD02686076
NACRES:	NA.41

Кат. номер

SAB4200211-25UL

SAB4200211-200UL

CPLA2/PLA2G4A (phospholipase A2, group IVA(cytosolic, calcium-dependent)) is located on human chromosome 1q31.1. cPLA2 is an 85 kDa protein made of 749 amino acids. It is a member of the the - hydrolase family. CPLA2 has an N-terminal C2 domain (Ca2+ - dependent, phospholipid binding) and two catalytic domains (/ hydrolase). This protein is located in the cytoplasm.
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Fragment mixture prepared by cleavage of DNA with Hind III.
The product is also available as DNA Molecular Weight Marker II, Digoxigenin-labeled.
Size Range: 0.12 to 23.1 kbp
Contents
Ready-to-use solution, 250 µg/mL, in TE buffer (Tris-HCl, 1 mM EDTA, pH 8.0)
Specificity
Anti-Phospholipase A2 (cPLA2) (N-terminal), specifically recognizes human cPLA2.
Anti-Phospholipase A2 (cPLA2) (N-terminal) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.
Biochem/physiol Actions
CPLA2/PLA2G4A (phospholipase A2, group IVA(cytosolic, calcium-dependent)) helps to hydrolyze phospholipids at the sn-2-acyl ester bond to generate free fatty acid and lysophospholipid second messengers. It is associated with several diseases like cardiovascular disease, diabetes, asthma and arthritis.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Storage and Stability
Store at 20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	~100 kDa
species reactivity	human
packaging	antibody small pack of 25 µL
concentration	~1.5 mg/mL
application(s)	immunoprecipitation (IP): 15-30 µg using HEK-293T cells over expressing human cPLA2","western blot: 1.5-3 µg/mL using extracts of HEK-293T cells over expressing human cPLA2
conjugate	unconjugated
UniProt accession no.	P47712,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... PLA2G4A(5321)

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable