Anti-Phospholipase A2 (CPLA2) antibody produced in rabbit

MDL number:	MFCD02686076
NACRES:	NA.41

Кат. номер

SAB4200210-25UL

SAB4200210-200UL

Cytosolic phospholipase A2, group IVa, (cPLA2, also known as cPLA2a, PLA2G4A) is a member of the PLA2 superfamily that catalyses the cleavage of fatty acids from the sn-2 position of phospholipids. PLA2 isoenzymes vary in their cellular localizations, Ca2+ sensitivities and substrate specificities. cPLA2 mRNA is widely expressed in tissues and in various cell types including platelets, macrophages and endothelial cells.
Anti-Phospholipase A2 (CPLA2) antibody produced in rabbit has been used in immunoblotting.
Biochem/physiol Actions
Cytosolic phospholipase A2 (cPLA2) catalyse the synthesis of precursors of proinflammatory mediators, such as prostaglandins and leukotrienes, through the release of arachidonic acid (AA) from membrane phospholipids. PLA2s play crucial roles in several cellular processes, including intracellular membrane trafficking, differentiation, proliferation and apoptosis. They are thought to play a role in oxidative and inflammatory responses in cerebral ischemia, Alzheimers disease (AD) and neuronal injury. Upon cell stimulation, cPLA2 is activated by increased intracellular Ca2+ levels and phosphorylation, resulting in its translocation from the cytosol to the endoplasmic reticulum and the nuclear membrane. cPLA2 is phosphorylated and activated by either ERK1/2, p38 MAPK or JNK at three sites, Ser505, Ser515 and Ser727, depending on the cell type and agonist.
synthetic peptide corresponding to amino acids 242-258 of human cPLA2 conjugated to KLH. The corresponding sequence is identical in mouse and rat cPLA2
Physical form
solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~100 kDa
species reactivity	human
packaging	antibody small pack of 25 µL
concentration	~1.5 mg/mL
application(s)	western blot: 2-4 µg/mL using extracts of HEK-293T cells over expressing human cPLA2
conjugate	unconjugated
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... cPLA2(5321)

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable