Anti-phospho TFEB (Ser142)

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABE1971-I-25UL

ABE1971-I-100UL

Transcription factor EB (UniProt: P19484; also known as Class E basic helix-loop-helix protein 35, bHLHe35) is encoded by the TFEB (also known as BHLHE35) gene (Gene ID: 7942) in human. TFEB is a basic Helix-Loop-Helix (bHLH) leucine zipper transcription factor that plays a major role in the regulation of lysosomal biogenesis. TFEB is also a main player in the transcriptional response to starvation and controls autophagy by positively regulating autophagosome formation and autophagosome-lysosome fusion. TFEB is phosphorylated by mTORC1 and ERK at Ser142, which prevents its nuclear translocation. Pharmacological inhibition of mTORC1, as well as starvation and lysosomal disruption, activates TFEB nuclear translocation. Similarly, the serine-threonine kinase RIP1/RIPK1 is shown to suppress basal autophagic flux by activating ERK, which in turn phosphorylates TFEB at Ser142 and thereby prevents TFEB-mediated transcription of gene products involved in autophagy.
Specificity
This rabbit polyclonal antibody specifically detects Transcription factor EB phosphorylated on Serine 142 in human cells.
KLH-conjugated linear peptide corresponding to 15 amino acids surrounding phosphoserine 142 from the N-terminal half of human Transcription factor EB, isoform 1.
Anti-phospho TFEB (Ser142), Cat. No. ABE1971-I, is a highly specific rabbit polyclonal antibody that targets Transcription factor EB phosphorylated on Serine 142 and has been tested for use in Western Blotting.
Research Category
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blotting in transfected HEK293 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho TFEB (Ser142) in lysate of HEK293 cells transfected with FLAG-tagged wild type TFEB.
Target description
~65 kDa observed; 52.87 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity Purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	human
species reactivity (predicted by homology)	mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology)
packaging	antibody small pack of 25 µL
application(s)	western blot: suitable
NCBI accession no.	NP_001161299,
UniProt accession no.	P19484,
Gene Information	human ... TFEB(7942)