Anti-phospho-p38 (Thr180/Tyr182) Antibody

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

9-272-25UL

9-272

9-272-100UL

Specificity
Recognizes p38α dual phosphorylation of Thr180 and Tyr182.
Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-p38α (Thr180/Tyr182).
Immunoblot Analysis: A 1:1,000 dilution of the lot detected p38 dually phosphorylated on residues Thr180 and Tyr182 in lysate from HeLa cells stimulated with anisomycin.
Immunfluorescence: A 1:100 dilution of this antibody detected p38 phosphorylated on both residues, Thr180 and Tyr182, in fixed HeLa cells stimulated with anisomycin.
Immunoprecipitation (IP): 2-5 µL (5-10 µL/mL) of this antibody immunoprecipitated both active p38 from anisomycin treated HeLa whole cell extract and human recombinant GST-p38, separately, in RIPA buffer.
Immunoprecipitation Kinase Reaction (IPK): 2-5 µL of this lot immunoprecipitated phosphorylated p38 from both anisomycin treated HeLa cells as well as recombinant p38, separately, and then placed into an in vitro Kinase reaction with recombinant GST-ATF-2 where it phosphorylated ATF-2 on Thr69/71.
Research Sub Category
MAP Kinases
This Anti-phospho-p38α (Thr180/Tyr182) Antibody is validated for use in IP, WB for the detection of phospho-p38α (Thr180/Tyr182).
Research Category
Signaling
Quality
Routinely evaluated by immunoblot.
Target description
38 kDa
Replaces: AB3828
Physical form
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.
ImmunoAffinity Purified
Storage and Stability
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Anisomycin treated HeLa cells
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	human, rat, mouse
packaging	antibody small pack of 25 µL
Торговая марка	Upstate®
application(s)	immunoprecipitation (IP): suitable","western blot: suitable
isotype	IgG
NCBI accession no.	NM_001315.1,","NM_139012.1,","NM_139013.1,","NM_139014.1,
UniProt accession no.	Q16539,
shipped in	dry ice
Gene Information	human ... MAPK14(1432)