Anti-phospho-MDA-5 (Ser104)

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABF192

ABF192-25UL

Interferon-induced helicase C domain-containing protein 1 (UniProt: Q9BYX4; also known as EC: 3.6.4.13, Clinically amyopathic dermatomyositis autoantigen 140 kDa, CADM-140 autoantigen, Helicase with 2 CARD domains, Helicard, Interferon-induced with helicase C domain protein 1, Melanoma differentiation-associated protein 5, MDA-5, Murabutide down-regulated protein, RIG-I-like receptor 2, RLR-2, RNA helicase-DEAD box protein 116) is encoded by the IFIH1 (also known as MDA5, RH116) gene (Gene ID: 64135) in human. MDA-5 is an innate immune receptor that acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and pro-inflammatory cytokines. MDA-5 is widely expressed, but at low levels. Expression is detected at slightly higher levels in placenta, pancreas and spleen. Its ligands include mRNA lacking 2-O-methylation at their 5 cap and long-dsRNA. In the absence of ligands it is monomeric and homodimerizes in the presence of dsRNA ligands. Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1), which activates the IKK-related kinases TBK1 and IKBKE that phosphorylate interferon regulatory factors IRF3 and IRF7. These factors activate transcription of antiviral immunological genes, including interferons alpha and beta. MDA-5 also plays an important role in enhancing natural killer cell function and is believed to be involved in growth inhibition and apoptosis in several tumor cell lines. MDA-5 contains 2 CARD domains; 1 helicase ATP-binding domain, 1 helicase C-terminal domain, and 1 RLR C-terminal regulatory domain.
Specificity
This rabbit polyclonal antibody specifically detects human MDA-5 protein phosphorylated on Ser104.
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to 12 amino acids surrounding Ser104 from the N-terminal region of human MDA-5.
Peptide Inhibition Analysis: A 1:500 dilution from a representative lot detected phospho-MDA-5 (Ser104) in Raji cell lysate.
Anti-phospho-MDA-5 (Ser104), Cat. No. ABF192 is a rabbit polyclonal antibody that detects MDA-5 phosphorylated on Ser104 and has been tested for use in Western Blotting and Peptide Inhibition Assay.
Research Category
Inflammation & Immunology
Quality
Evaluated by Western Blotting in Daudi cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-MDA-5 (Ser104) in 10 µg of Daudi cell lysate.
Target description
~117 kDa observed; 116.69 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4 with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	human
packaging	antibody small pack of 25 µL
application(s)	inhibition assay: suitable (peptide)","western blot: suitable
NCBI accession no.	NP_071451.2,
UniProt accession no.	Q9BYX4,
shipped in	ambient

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable