Anti-phospho-AKT1 Antibody, Thr479

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABS1526

ABS1526-25UG

RAC-alpha serine/threonine-protein kinase (UniProt: P31749; EC: 2.7.11.1; Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1) is encoded by the AKT1 (also known as PKB, RAC) gene (Gene ID: 207) in human. AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) that regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. In mammals these 3 isoforms of AKT exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. AKT1 is constitutively phosphorylated on Ser124, in the region between PH and catalytic domains, and on Thr450, in the C-terminal region in unstimulated cells. Phosphorylation at Thr308 and Ser473 induces complete activation of AKT1. AKT1 contains one PH domain (aa 5-108), one protein kinase domain (aa 150-408), and one AGC-kinase C-terminal domain (aa 409-480). Binding of the PH domain to phosphatidylinositol 3,4,5-trisphosphate (PIP3) results in its targeting to the plasma membrane. AKT1 can be phosphorylated at Ser477/Thr479 by Cdk2/Cyclin A, which locks it into an active conformation. Ser477/Thr479 phosphorylation is believe to trigger Akt1 activation either through enhancing the association between AKT1 and mTORC2 to promote phosphorylation of Ser473, or by functionally compensating for phosphorylated Ser473 to lock AKT1 in its active conformation. AKT1 phosphorylated at Ser477/Thr479 is reported to display increased association with Sin1 and mTOR complexes. (Ref.: Liu, P et al (2014). Nature. 508(7497): 541-545).
Specificity
This rabbit polyclonal antibody detects AKT1 phosphorylated on Thr479.
KLH-conjugated linear peptide corresponding to 10 amino acids surrounding Thr479 from the C-terminal region of human AKT1.
Peptide Inhibition Analysis: A 1:500 dilution from a representative lot detected phospho-AKT1, Thr479 in RAT2 cells.
Research Category
Signaling
Anti-phospho-AKT1, Thr479, Cat. No. ABS1526, is a rabbit polyclonal antibody that detects AKT1 phosphorylated on Thr479 and has been tested for use in Western Blotting and Peptide Inhibition Assay.
Quality
Evaluated by Western Blotting in RAT2 cells transfected with Src (21-154).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-AKT1, Thr479 in lysate of RAT2 cells transfected with Src (21-154).
Target description
~56 kDa observed; 55.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	rat
species reactivity (predicted by homology)	mouse (based on 100% sequence homology), human (based on 100% sequence homology)
packaging	antibody small pack of 25 µg
application(s)	inhibition assay: suitable (peptide)","western blot: suitable
NCBI accession no.	NP_001014431.1,
UniProt accession no.	P31749,
shipped in	ambient

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable