Anti-PHD finger protein 14 (PHF14)

eCl@ss:

32160702

Кат. номер

ABE1359-25UL

ABE1359-100UL

PHD finger protein 14 (UniProt: O94880) is encoded by the PHF14 (also known as KIAA0783) gene (Gene ID: 9678) in human. PHF14 is a member of the PHD finger protein family that serves as a chromatin-binding protein. It contains four putative PHD fingers, two coiled-coil regions, and four zinc fingers. PHF14 is highly conserved from C. elegans to humans and is considered as a transcriptional regulator of target gene expression. PHF14 has been identified as a regulator of mesenchyme growth in embryonic tissues. Two isoforms of PHF14 have been described that are produced by alternative splicing. PHF14 interacts with histones via its PHD1 and PHD3 domains indicating its role in epigenetic regulation. It is involved in chromatin-mediated transcriptional regulation and chromatin dynamics. During cell division, PHF14 is shown to be directly bound to and co-localized with KIF4A to form a functional complex. PHF14 is shown to be overexpressed in lung cancer and this overexpression is associated with poor prognosis of non-small cell lung carcinoma. (Ref.: Zhang, L., et al. (2017). Oncotarget. 8(12); 19684-19698; Yang, B., et al. (2017). Sci Rep. 7; 39888).
Specificity
This rabbit polyclonal antibody detects PHD Finger Protein 14 in human cells. It targets an epitope within 16 amino acids from the N-terminal half.
KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal half of human PHD finger protein 14.
Anti-PHD finger protein 14 (PHF14) Antibody, Cat. No. ABE1359, is a highly specific rabbit polyclonal antibody that detects PHD finger protein 14 and has been tested for use in Immunocytochemistry and in Western Blotting.
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected PHD finger protein 14 (PHF14) in HeLa cells.
Quality
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected PHD finger protein 14 (PHF14) in HEK293 cell lysate.
Target description
100.0 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	human
species reactivity (predicted by homology)	mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
packaging	antibody small pack of 25 µL
application(s)	immunocytochemistry: suitable","western blot: suitable
NCBI accession no.	NP_055475.2,
UniProt accession no.	O94880,
Gene Information	human ... PHF14(9678)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable