Anti-PDGF-C (GFD)

eCl@ss:

32160702

Кат. номер

ABC1392-25UG

ABC1392-100UG

Platelet-derived growth factor C (UniProt: Q9NRA1; also known as PDGF-C, Fallotein, Spinal cord-derived growth factor, SCDGF, VEGF-E) is encoded by the PDGFC (also known as SCDGE, UNQ174/PRO200) gene (Gene ID: 56034) in human. PDGF-C is a disulfide-linked homodimeric growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. It also plays an important role in wound healing and angiogenesis. It also serves as a potent mitogen and chemoattractant for cells of mesenchymal origin. It is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off in the mature form. Its CUB domain is localized to amino acids 46 to 163 and proteolytic removal of the CUB domain is essential for unmasking the receptor-binding epitopes of the core domain. PDGF-C is expressed in the fallopian tube, vascular smooth muscle cells in kidney, breast and colon and in visceral smooth muscle of the gastrointestinal tract. Highly expressed in retinal pigment epithelia. Its high expression is also reported in retinal pigment epithelia. In the kidney, it is constitutively expressed in parietal epithelial cells of Bowmans capsule, tubular epithelial cells, and in arterial endothelial cells. Higher levels of PDGF-C and have been reported in the malignant breast tumor microenvironment. In MCF-7 cells increased expression of PDGF-C is reported to enhance cell proliferation and tumor cell motility. Tissue plasminogen activator and matriptase are shown to process PDGF-C in MCF-7 cells to its active form. (Ref.: Hurst, NJ., et al. (2012). Biochem. J. 441(3); 909-918; Lei, H., and Kazlauskas, A (2008). Exp. Eye Res. 86(5); 711-712).
Specificity
This rabbit polyclonal antibody detects full length platelet derived growth factor C (PDGF-C) and also the 18 kDa growth factor domain.
A linear peptide corresponding to 21 amino acids from the C-terminal growth factor domain of human Platelet-derived growth factor C (PDGF-C).
Epitope: C-terminus
Anti-PDGF-C, Cat. No. ABC1392, is a rabbit polyclonal antibody that detects Platelet-derived growth factor C and has been tested for use in Western Blotting.
Research Category
Signaling
Western Blotting Analysis: A representative lot detected PDGF-C in Western Blotting applications (Hurst, N.J., et. al. (2012). Biochem J. 441(3):909-18).
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected PDGF-C in HEK293T cells. (Courtesy of Hyeong-Reh Choi Kim lab at Wayne State University).
Quality
Evaluated by Western Blotting in HEK293T cells.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected PDGF-C in lysates from HEK293T cells.
Target description
~48 kDa observed; 39.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
species reactivity	human
species reactivity (predicted by homology)	monkey (based on 100% sequence homology)
packaging	antibody small pack of 25 µg
application(s)	western blot: suitable
isotype	IgG
NCBI accession no.	NP_057289,
UniProt accession no.	Q9NRA1,
Gene Information	human ... PDGFC(56034)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable