Anti-PD2 (PAF1) Antibody, clone F169-3B2

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

MABT1495-25UG

MABT1495-100UG

Pancreatic differentiation protein 2 (UniProt: Q8N7H5; also known as RNA polymerase II-associated factor 1 homolog, PD2, hPAF1) is encoded by the PAF1 (also known as PD2) gene (Gene ID: 54623) in human. PD2 is a nuclear protein that serves as a subunit of the RNA polymerase II associated factor (PAF) complex. It has multiple functions during transcription by RNA polymerase II. It has been implicated in regulation of development and maintenance of embryonic stem cell pluripotency. It associates with RNA polymerase II through interaction with POLR2A C-terminal domain non-phosphorylated and serine 2 and serine 5 phosphorylated forms. It is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. Three isoforms of PD2 have been described that are produced by alternative splicing. PD2 has oncogenic property and is shown to be overexpressed in pancreatic cancer cells. In normal murine pancreas, its expression is restricted to acinar cells, however, its expression is increased in the ductal cells of mouse model of pancreatic cancer with advancing age. (Ref.: Dey, P., et al (2014). Oncotarget, 5(12); 4480-4491).
Specificity
Clone F169-3B2 detects Pancreatic differentiation protein 2 (PD2) in human. It targets an epitope with in 22 amino acids from the C-terminal half.
KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal half of human Pancreatic differentiation protein 2 (PD2).
Anti-PD2 (PAF1), clone F169-3B2, Cat. No. MABT1495, is mouse monoclonal antibody that detects Pancreatic differentiation protein 2 and has been tested for use in ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Immunocytochemistry Analysis: A representative lot detected PD2 (PAF1) in Immunocytochemistry applications (Dey, P., et. al. (2014). Oncotarget. 5(12):4480-91; Moniaux, N., et. al. (2006). Oncogene. 25(23):3247-57).
Immunoprecipitation Analysis: A representative lot immunoprecipitated PD2 (PAF1) in Immunoprecipitation applications (Moniaux, N., et. al. (2006). Oncogene. 25(23):3247-57).
ELISA Analysis: A representative lot detected PD2 (PAF1) in ELISA applications (Moniaux, N., et. al. (2006). Oncogene. 25(23):3247-57).
Immunohistochemistry Analysis: A representative lot detected PD2 (PAF1) in Immunohistochemistry applications (Dey, P., et. al. (2014). Oncotarget. 5(12):4480-91).
Western Blotting Analysis: A representative lot detected PD2 (PAF1) in Western Blotting applications (Moniaux, N., et. al. (2006). Oncogene. 25(23):3247-57).
Research Category
Epigenetics & Nuclear Function
Quality
Evaluated by Immunocytochemistry in Panc1 cells.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected PD2 (PAF1) in Panc1 cells.
Target description
59.98 kDa calculated.
Physical form
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	F169-3B2, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	ELISA: suitable","immunocytochemistry: suitable","immunohistochemistry: suitable","immunoprecipitation (IP): suitable","western blot: suitable
isotype	IgG1
NCBI accession no.	NP_061961,
UniProt accession no.	Q8N7H5,