Anti-PD-L2 Antibody, clone 366C.9E5

eCl@ss:

32160702

Кат. номер

MABC1120-25UG

MABC1120-100UG

Programmed cell death 1 ligand 2 (UniProt: Q9BQ51; also known as PD-1 ligand 2, PD-L2, PDCD1 ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, B7-DC, CD273) is encoded by the PDCD1LG2 (also known as B7DC, CD273, PDCD1L2, PDL2) gene (Gene ID: 80380) in human. Three isoforms of PD-L2 have been reported that are produced by alternative splicing. Isoforms 1 and 2 are single-pass type I membrane proteins and isoform 3 is a secreted protein. PD-1 and PD-1 ligands 1 and 2 (PD-L1 and PD-L2) are B7:CD28 family members that regulate T cell activation and peripheral tolerance. When engaged together with the TCR, the interaction of PD-1 with its ligands delivers an inhibitory signal to T cell proliferation and cytokine production. While PD-L1 is broadly expressed in hematopoietic and non-hematopoietic cells, PD-L2 expression is highly restricted to antigen presenting cells (APCs), including dendritic cells (DCs) and macrophages. The PD-1 pathway plays a key role in the progressive loss of effector T cell responses during chronic HIV infection. Under some conditions, blockade of this pathway can restore many T cell functions. PD-L2 is initially produced with signal peptide (aa 1-19) that is removed to produce the mature protein that contains a large extracellular region (aa. 20-220), a transmembrane domain (aa 221-241), and a cytoplasmic tail (aa 242-273).
Specificity
Clone 366C.12.9E5.E10.19.1F8 specifically detects PD-L2 in human cells. It targets an epitope within the extracellular domain.
Recombinant protein fragment corresponding to the extracellular domain of human PD-L2 (as an Ig fusion protein).
Epitope: extracellular domain
Research Category
Apoptosis & Cancer
Anti-PD-L2, clone 366C.9E5, Cat. No. MABC1120, is a mouse monoclonal antibody that detects Programmed cell death 1 ligand 2 (PD-L2) and has has been tested for use in Immunohistochemistry (Paraffin).
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected PD-L2 in human prostate tissue.
Immunohistochemistry Analysis: A representative lot detected PD-L2 in Immunohistochemistry applications (Sridharan, V., et. al. (2016). Cancer Immunol Res. 4(8):679-87; Roemer, M.G., et. al. (2016). J Clin Oncol. 34(23):2690-7; Chong, L.C., et. al. (2016). Blood. 128(9):1206-13; Calles, A., et. al. (2015). J Thorac Oncol. 10(12):1726-35).
Quality
Evaluated by Immunohistochemistry (Paraffin) in human tonsil tissue.
Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected PD1-L2 in human tonsil tissue.
Target description
30.96 kDa calculated.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	366C.12.9E5.E10.19.1F8, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	immunohistochemistry: suitable (paraffin)
isotype	IgG1
NCBI accession no.	NP_079515,
UniProt accession no.	Q9BQ51,