Anti-PCBP2 (hnRNP E2) Antibody, clone PC81-1

eCl@ss:

32160702

Кат. номер

MABF2122-25UG

MABF2122-100UG

Poly(rC)-binding protein 2 (UniProt: Q15366; also known as Alpha-CP2, Heterogeneous nuclear ribonucleoprotein E2, hnRNP E2) is encoded by the PCBP2 gene (Gene ID: 5094) in human. PCBP2 belongs to a class of cellular proteins that bind to poly(C) stretches of both RNA and DNA and has been detected in all tissues studied. PCBP2 shuttles between the nucleus and cytoplasm and exerts its impact on RNA processing and mRNA expression through sequence-specific interactions with C-rich determinants within target mRNAs. It acts as an important mediator of multiple processes, including replication of viruses with gastrointestinal and hepatic tropism, hepatic collagen synthesis, globin expression, and cellular proliferation. PCBP2 is shown to negatively regulate cellular antiviral responses mediated by mitochondrial antiviral-signaling protein (MAVS) signaling and acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, thereby triggering MAVS ubiquitination and degradation. Together with PCBP1, it is shown to function as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. PCBP2 contains three K-homologous (KH) domains that mediates poly(C) binding. Eight different isoforms of PCBP2 have been described that are produced by alternative splicing. (Ref.: Ghanem, LR et al. (2014). Gene Exp. Patterns 14(2); 78-87; Sean, P., et al. (2008). Virology. Virology. 378(2); 243-253).
Specificity
Clone PC 81-1 specifically detects Poly(rC)-binding protein 2 in human cells.
His-tagged full-length human recombinant Poly(rC)-binding protein 2.
Western Blotting Analysis: A representative lot detected PCBP2 (hnRNP E2) in Western Blotting applications (Sean, P., et. al. (2008). Virology. 378(2):243-53; Chase, A.J., et. al. (2014). Virology. 449:35-44).
Immunocytochemistry Analysis: A representative lot detected PCBP2 (hnRNP E2) in Immunocytochemistry applications (Fitzgerald, K.D., et. at. (2011). PLoS Pathog. 7(7):e1002127).
Western Blotting Analysis: 1 µg/mL from a representative lot detected PCBP2 (hnRNP E2) in SK-N-SH cell lysate.
Anti-PCBP2 (hnRNP E2), clone PC81-1, Cat. No. MABF2122, is a mouse monoclonal antibody that detects Poly(rC)-binding protein 2 and has been tested for use in Immunocytochemistry and Western Blotting.
Research Category
Inflammation & Immunology
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected PCBP2 (hnRNP E2) in HeLa cell lysate.
Target description
~38 kDa observed; 38.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	PC 81-1, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	immunocytochemistry: suitable","western blot: suitable
isotype	IgG2b
NCBI accession no.	NP_001122383,
UniProt accession no.	Q15366,
Gene Information	human ... PCBP2(5094)