Anti-Pancreatic Polypeptide

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

AB939-I

AB939-I-25UL

Pancreatic polypeptide (UniProt: P01298; also known as PP, Obinepitide, Pancreatic prohormone) is encoded by the PPY (also known as PNP) gene (Gene ID: 5539) in human. Pancreatic polypeptide is a member of the neuropeptide Y family and is secreted protein that is produced by PP cells in the pancreatic islets of Langerhans. It is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off. It acts as a regulator of pancreatic and gastrointestinal functions. Two isoforms of pancreatic polypeptide have been described that are generated by alternative splicing. Pancreatic polypeptide release is functionally associated with increased satiety, delayed gastric emptying, inhibition of gallbladder motility, and regulation of energy expenditure. Its deficiency is observed in cases of pancreatic resection and chronic pancreatitis and is associated with significant reduction in PP cell mass and abnormally high hepatic glucose production even in the presence of endogenous insulin. (Ref.: Maxwell, J et al. (2014). Pancreas 43(4); 651-656).
Specificity
This rabbit polyclonal antibody detects both isoforms of human pancreatic polypeptide. It targets an epitope within 36 amino acids from the internal region.
Epitope: cytoplasmic domain
KLH-conjugated linear peptide corresponding to 36 amino acids the internal region of human pancreatic polypeptide.
Western Blotting Analysis: 1:2,000 dilution from a representative lot detected Pancreatic Polypeptide in 10 µg of human pancreas tissue lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Pancreatic Polypeptide in human pancreas tissue sections.
Immunocytochemistry Analysis (ICC): A 1:500 dilution from a representative lot detected Pancreatic Polypeptide in TGP52 cell line.
Research Category
Neuroscience
Anti-Pancreatic Polypeptide Antibody, Cat. No. AB939-I, is a highly specific rabbit polyclonal antibody that targets Pancreatic polypeptide and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Quality
Evaluated by Western Blotting in PANC-1 cell lysate.
Western Blotting Analysis: 1:2,000 dilution of this antibody detected Pancreatic Polypeptide in 10 µg of PANC-1 cell lysate.
Target description
~11 and ~20 kDa observed for isoforms 1 and 2; 10.45 and 18.79 kDa calculated for isoforms 1 and 2. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
species reactivity	human
species reactivity (predicted by homology)	rhesus macaque (based on 100% sequence homology)
packaging	antibody small pack of 25 µL
application(s)	immunohistochemistry: suitable (paraffin)","western blot: suitable
NCBI accession no.	NM_002722.3,
UniProt accession no.	P01298,
shipped in	ambient
Gene Information	human ... PPY(5539)