Anti-pan-Actin Antibody, clone 2A3

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

MABT1333-25UL

MABT1333-100UL

Actin is a multi-functional cytoskeletal protein that serves as the basic building block of cytoskeletal microfilaments. Six different actin types have been reported. Skeletal muscle alpha-actin (UniProt: P68133) is encoded by the ACTA1 gene, smooth muscle alpha-actin (UniProt: P62736) by the ACTA2 gene, cytoplasmic beta-actin (UniProt: P60709) by the ACTB gene, cardiac muscle alpha-actin (UniProt: P68032) by the ACTC1 gene, cytoplasmic gamma-actin (UniProt: P63261) by the ACTG1 gene, and smooth muscle gamma-actin (UniProt: P63267) by the ACTG2 (also known as ACTA3) gene. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic beta and gamma actins are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt. This pan-actin antibody detects all isoforms actin.
Specificity
Clone 2A3 detects all forms of actin in multiple species. It targets an epitope with in the C-terminal region.
KLH-conjugated linear peptide corresponding to a fragment from the C-terminal region of human Actin.
Research Category
Cell Structure
Anti-pan-Actin, clone 2A3, Cat. No. MABT1333, is a mouse monoclonal antibody that detects all forms of actin and has been tested for use in Western Blotting.
Western Blotting Analysis: A 1:50 dilution from a representative lot detected Actins in various species samples (e.g. Human, rat, mouse, monkey, Hamster, Drosophila, Yeast) (Courtesy of Martina Mitterhuber, M.sc and Egon Ogris, M.D., Medical University of Vienna, Austria).
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Actins in HeLa cell lysate.
Target description
~42 kDa observed; 41.74 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	2A3, monoclonal
species reactivity	mouse, Drosophila, hamster, rat, human, yeast, monkey
packaging	antibody small pack of 25 µL
application(s)	western blot: suitable
isotype	IgG1
NCBI accession no.	NP_001092,
UniProt accession no.	P60709,
Gene Information	human ... ACTA1(58), ACTC1(70), ACTG1(71)