Anti-Palladin (C-terminal) antibody produced in rabbit

NACRES:

NA.41

Кат. номер

A3861-200UL

A3861-25UL

Anti-Palladin (C-terminal) is produced in rabbit using as the immunogen a synthetic peptide corresponding to a sequence at the C-terminal of human palladin, conjugated to KLH. Palladin, an actin associated Ig-domain protein is mapped to human chromosome 4q32. This phosphoprotein is widely expressed in vertebrate cells and tissues. Palladin is expressed as three major isoforms of 90, 140 and 200 kDa.
Rabbit anti-Palladin (C-terminal) antibody can be used for immunoprecipitation (10-20μL), indirect immunofluorescence (1:250-1:500) and western blot (1:250 -1:500) using HeLa cells.
Anti-Palladin (C-terminal) antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions
Palladin is a component of actin-containing microfilaments that control cell shape, adhesion, and contraction. Its expression is required for normal mammalian embryogenesis since inactivation of paladin leads to embryonic lethality. Palladin interacts with the actin-associated proteins -actinin, VASP, ezrin, and profilin, suggesting that it is required for normal actin cytoskeleton organization. It serves as a transcriptional regulator, which controls the expression of smooth muscle cell genes.
Palladin is a microfilament-associated phosphoprotein that is known to colocalize with alpha-actinin at cell-cell junctions, stress fibres, embryonic Z-lines, and focal adhesions. Palladin abnormalities have been linked to familial pancreatic cancer. Rabbit anti-Palladin (C-terminal) antibody binds to human palladin. Detection of the palladin band by immunoblotting is specifically inhibited by the immunizing peptide. A nonspecific band of approx. 55kDa may be detected in some cell extract preparations.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	IgG fraction of antiserum
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~140 kDa (another band at 90 kDa)
species reactivity	human
packaging	antibody small pack of 25 µL
application(s)	immunoprecipitation (IP): 10-20 µL using HeLa cell lysates","indirect immunofluorescence: 1:250-1:500 using human HeLa cells","western blot: 1:250-1:500 using whole extracts of human HeLa cells
conjugate	unconjugated
UniProt accession no.	Q8WX93,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... PALLD(23022) mouse ... Palld(72333)

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable