Anti-p15 Antibody, clone PAT56B

eCl@ss:

32160702

Кат. номер

MABS1950-25UG

MABS1950-100UG

Cyclin-dependent kinase 4 inhibitor B (UniProt: P55271; also known as p14-INK4b, p15-INK4b) is encoded by the Cdkn2b gene (Gene ID: 12579) in murine species. p15-INK4b is a ubiquitously expressed member of the INK4 family of cyclin-dependent kinase inhibitors. It induces a G1-phase cell cycle arrest through heterodimerization with cyclin-dependent kinase 4/6, thereby serves as a tumor suppressor. It also serves as a potential effector of TGF-beta induced cell cycle arrest. p15 also plays an important role in the regulation of cellular commitment of hematopoietic progenitor cells and myeloid cell differentiation. It contains four ankyrin repeats (aa 5-34; 38-66; 71-100; and 104-130). p15 shares extensive homology with p16 and serves an important backup function for p16 in senescence. Cdkn2b gene is frequently hypermethylated in myeloid neoplasia. Genes coding for both p15 and p16 are reported to often deleted in human tumors. p15 can serve as a good prognostic marker for monitoring the response to treatment with DNA methylation inhibitors. (Ref.: Wolff, L., and Bies, J. (2013). Blood Cells Mol. Dis. 50(4); 227-231).
Specificity
Clone PAT56B is a rat monoclonal antibody that specifically detects murine Cyclin-dependent kinase 4 inhibitor B (p15-INK4b).
GST/His-tagged recombinant full-length mouse Cyclin-dependent kinase 4 inhibitor B (p15-INK4b).
Anti-p15, clone PAT56B, Cat. No. MABS1950, is a rat monoclonal antibody that detects Cyclin-dependent kinase 4 inhibitor B (p15-INK4b) and has been tested for use in Immunohistochemistry and Western Blotting.
Research Category
Signaling
Immunohistochemistry Analysis: A representative lot detected p15 in Immunohistochemistry applications (Munoz-Espin, D., et. al. (2013). Cell. 155(5):1104-18).
Quality
Evaluated by Western Blotting in NIH/3T3 cell lysates.
Western Blotting Analysis: 4 µg/mL of this antibody detected p15 in NIH/3T3 cell lysates.
Target description
~14 kDa observed; 13.79 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rat
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	PAT56B, monoclonal
species reactivity	mouse
packaging	antibody small pack of 25 µg
application(s)	immunohistochemistry: suitable","western blot: suitable
isotype	IgG2a
NCBI accession no.	NP_031696.1,
UniProt accession no.	P55271,

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable