Anti-Nurr-1

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABN1675

ABN1675-25UL

Nuclear receptor subfamily 4 group A member 2 (UniProt: P43354; also known as Immediate-early response protein NOT, Orphan nuclear receptor NURR1, Transcriptionally-inducible nuclear receptor) and is encoded by the NR4A2 (also known as NOT, NURR1, TINUR) gene (Gene ID: 4929) in human. Nurr-1 belongs to the nuclear hormone receptor family that acts as a transcriptional regulator and is considered to be important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is also crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. It is expressed in a number of T cell, B cell and fibroblast cell lines, but its expression is higher in brain tissue. Two isoforms of Nurr-1 have been reported that are produced by alternative splicing. The ligand-binding domain (LBD) of Nurr-1 contains no cavity as a result of the tight packing of side chains from several bulky hydrophobic residues in the region normally occupied by ligands. It also lacks a classical binding site for co-activators. However, it has hydrophobic region on the surface of LBD, which is critical for its activity. (Ref.: Volakakis N et al (2006). J. Mol. Endocrinol 37(2); 317-326; Wang G et al. (2003). Nature 423(6939):555-560).
Specificity
This rabbit polyclonal antibody detects Nurr-1 in human and murine tissues. It targets an epitope within the C-terminal half.
His-tagged recombinant fragment corresponding to 271 amino acids from the C-terminal half of human Nurr-1 and includes the ligand binding domain.
Epitope: domain of:
Anti-Nurr-1, Cat. No. ABN1675, is a highly specific rabbit polyclonal antibody that targets Nuclear receptor subfamily 4 group A member 2 and has been tested in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
Research Category
Neuroscience
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Nurr-1 in mouse hippocampus and human cerebellum tissue sections.
ELISA Analysis: A representative lot detected recombinant Nurr-1 LBDs (Courtesy of Dr. Pierre Leblanc at McLean Hospital, Belmont, MA).
Immunohistochemistry Analysis: A representative lot detected Nurr-1 in Midbrain dopamine neurons (Leblanc, P., et. al. (2015). J Vis Exp. (102):e52963).
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected Nurr-1 in HEK293T and CHO cells overexpressed with Myc-Tagged mouse full length Nurr1/Nor1/Nurr77 (Courtesy of Dr. Pierre Leblanc at McLean Hospital, Belmont, MA).
Immunohistochemistry Analysis: A 1:250-1:2,500 dilution from a representative lot detected Nurr-1 in mouse brain tissue sections.(Courtesy of Dr. Pierre Leblanc at McLean Hospital, Belmont, MA).
ELISA Analysis: A representative lot detected Nurr-1 ligand binding domain (Leblanc, P., et. al. (2015). J Vis Exp. (102):e52963).
Western Blotting Analysis: A representative lot detected Nurr-1 in CHO cells transfected with full-length myc-tagged Nurr-1 (Leblanc, P., et. al. (2015). J Vis Exp. (102):e52963).
Quality
Evaluated by Immunohistochemistry in mouse cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Nurr-1 in mouse cerebral cortex tissue sections.
Target description
~70 kDa observed; 66.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Unpurified
Unpurified
Rabbit polyclonal antiserum in PBS with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	unpurified
antibody product type	primary antibodies
clone	polyclonal
species reactivity	mouse, human
species reactivity (predicted by homology)	rat (based on 100% sequence homology)
packaging	antibody small pack of 25 µL
application(s)	ELISA: suitable","immunohistochemistry: suitable (paraffin)","western blot: suitable
isotype	IgG
NCBI accession no.	NP_006177,
UniProt accession no.	P43354,
Gene Information	human ... NR4A2(4929)

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable