Anti-NUDT21

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABE2873

ABE2873-25UL

Cleavage and polyadenylation specificity factor subunit 5 (UniProt: O43809; also known as Cleavage and polyadenylation specificity factor 25 kDa subunit, CFIm25, CPSF 25 kDa subunit, Nucleoside diphosphate-linked moiety X motif 21, Nudix motif 21, Pre-mRNA cleavage factor Im 25 kDa subunit) is encoded by the NUDT21 (also known as CFIM25, CPSF25, CPSF5) gene (Gene ID: 11051) in human. CFlm25 is a homodimeric protein that is a component of the cleavage factor Im (CFIm) complex, which plays a key role in pre-mRNA 3-processing. It is involved in association with CPSF6 or CPSF7 in pre-MRNA 3-end poly(A) site cleavage and poly(A) addition. The interaction of CFlm25 with the RNA is one of the earliest steps in the assembly of the 3 end processing complex and facilitates the recruitment of other processing factors. It contains one Nudix hydrolase domain (aa 76-201). The region containing aa 2-147 is considered to be essential for RNA-binding. It can be acetylated by p300/CBP and acetylation results in a reduction in its interaction with Poly(A) polymerase alpha (PAPAO).
Specificity
This rabbit polyclonal antibody detects Cleavage and polyadenylation specificity factor subunit 5 (CFIm25) in human cells.
His-tagged full length recombinant human Cleavage and polyadenylation specificity factor subunit 5 (CFIm25).
Epitope: unknown
Anti-NUDT21, Cat. No. ABE2873, is a highly specific rabbit polyclonal antibody that targets Cleavage and polyadenylation specificity factor subunit 5 and has been tested in Immunoprecipitation and Western Blotting.
Western Blotting Analysis: A representative lot detected NUDT21 in RNase A-treated HeLa whole-cell extract after IP (Katahira, J., et. al. (2013). Nucleic Acid Res. .41(14):7060-72).
Immunoprecipitation Analysis: A representative lot detected NUDT21 in Co-immunoprecipitation with FLAG-tagged CF I cell lysates (Shimazu, T., et. al. (2007). J Biol Chem. 282(7):4470-8).
Research Category
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blotting in Caco-2 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected NUDT21 in 10 µg of Caco-2 cell lysate.
Target description
~25 kDa observed; 26.23 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
species reactivity	human
packaging	antibody small pack of 25 µL
application(s)	immunoprecipitation (IP): suitable","western blot: suitable
isotype	IgG
NCBI accession no.	NP_008937,
UniProt accession no.	O43809,
shipped in	ambient
Gene Information	human ... NUDT21(11051)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable