Anti-NRP1

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABC1469

ABC1469-25UL

Neuropilin-1 (UniProt: O14786; also known as Vascular endothelial cell growth factor 165 receptor, CD304) is encoded by the NRP1 (also known as NRP, VEGF165R) gene (Gene ID: 8829) in human. Three isoforms of Neuropilin-1 have been reported that are produced by alternative splicing. Isoform 1 of Neuropilin-1 is a homodimer, single-pass type I membrane protein with three calcium-binding sites (aa 195, 209, and 250). It can also form heterodimers with neuropilin-2. This membrane-bound isoform serves as a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF165 isoform of VEGFA and VEGFB. Its co-expression with VEGFR-2 (KDR) results in increased VEGF165 binding to VEGFR-2 as well as increased chemotaxis. It has an extracellular domain (aa 22-856), a helical domain (aa 857-879), and the cytoplasmic domain (aa 880-923). It is produced with a signal peptide (aa 1-21) that is subsequently cleaved to generate the mature form. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. It may also bind various members of the semaphorin family and its expression has an adverse effect on blood vessel number and integrity. The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.
Specificity
This rabbit polyclonal antibody detects Neuropilin-1 in human cells. It targets an epitope within 313 amino acids from the internal region.
His-tagged recombinant fragment corresponding to 313 amino acids from the internal region of human Neuropilin-1.
Anti-NRP1, Cat. No. ABC1469, is a rabbit polyclonal antibody that detects human Neuropilin-1 and has been tested for use in Immunohistochemistry (Paraffin), Inhibition Activity/Function, and Western Blotting.
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected NRP1 in human prostate cancer and human bone marrow tissue.
Western Blotting Analysis: A representative lot detected NRP1 in Western Blotting applications (Pang, H.B., et. al. (2014). Nat Commun. 5:4904).
Inhibits Activity/Function: A representative lot reduced iRGD-induced vascular permeability in tumor tissue and reduced the uptake of nanoparticles. (Pang, H.B., et. al. (2015). Sci Adv. 1(10):e1500821; Sugahara, K.N., et. al. (2010). Science. 328(5981):1031-5).
Quality
Evaluated by Western Blotting with recombinant Neuropilin-1.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected recombinant Neuropilin-1. recombinant protein.
Target description
~36 kDa observed for recombinant fragment. Uncharacterized bands may be observed in some lysate(s).
Concentration: Please refer to lot specific datasheet.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	human
packaging	antibody small pack of 25 µL
application(s)	immunohistochemistry: suitable (paraffin)","western blot: suitable
isotype	IgG
NCBI accession no.	NP_003864,
UniProt accession no.	O14786,
shipped in	ambient
Gene Information	human ... NRP1(8829)

Flash Point F	does not flash
Flash Point C	does not flash