Anti-Nexilin antibody,Mouse monoclonal

Кат. номер

SAB4200124-25UL

SAB4200124-200UL

Monoclonal Anti-Nexilin (mouse IgG2a isotype) is derived from the hybridoma NX-38 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide conjugated to KLH. Nexilin is a filamentous actin-binding protein that localizes to focal contacts. Alternative splicing of the gene yields two different isoforms namely, b-nexilin and s-nexilin.
NEXN (nexilin), a cardiac Z-disc protein is made of 675 amino acids. It is present in the heart and skeletal muscle. NEXN is mapped to human chromosome 1p31.
Specificity
Monoclonal Anti-Nexilin recognizes human, rat, and mouse nexilin.
Anti-Nexilin antibody,Mouse monoclonal may be used in immunoblotting and immunocytochemistry.
Biochem/physiol Actions
NEXN (nexilin) plays an important role in protecting cardiac Z-discs from forces generated within the sarcomere. Mutations in NEXN results in hypertrophic cardiomyopathy.
Nexilin may be involved in cell adhesion and migration.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified from hybridoma cell culture
antibody product type	primary antibodies
clone	NX-38, monoclonal
form	buffered aqueous solution
mol wt	antigen 80 kDa
species reactivity	rat, human, mouse
packaging	antibody small pack of 25 µL
concentration	~1 mg/mL
application(s)	immunocytochemistry: suitable","western blot: 1-2 µg/mL using using rat brain S1 fraction extracts
isotype	IgG2a
conjugate	unconjugated
UniProt accession no.	Q0ZGT2,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... NEXN(91624) rat ... Nexn(246172), Nexn(68810)

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable