Anti- Neurofilament 200-FITC antibody, Mouse monoclonal

Кат. номер

SAB4200811-25UL

SAB4200811-100UL

Neurofilaments are type of intermediate filaments (IFs) that serve as major elements of the cytoskeleton supporting the axon cytoplasm of neuronal cells. IFs are components of most eukaryotic cells and significantly differ from other cytoskeletal elements of the cell, namely microtubules and microfilaments.
Specificity
Monoclonal Anti-Neurofilament 200, also known as Neurofilament-H or Heavy subunit, specifically recognizes the phosphorylated H tail of Neurofilament 200 and shows no reactivity on enzymatically dephosphorylated neurofilaments.2 The antibody shows reactivity with neurofilaments in the central and peripheral nervous systems from human1, pig1, mouse3, rat4, chicken3, guinea pig3, feline3 and bovine3 origin.
Neurofilaments purified from pig spinal cord
The antibody may be used in various immunochemical techniques including Immunohistochemistry and Immunoblotting (~200 kDa).1-7
Biochem/physiol Actions
Neurofilaments undergo post-translational modifications including different levels of phosphorylation, which has been suggested to modulate their function by influencing the interaction between neurofilament and cytoplasmic organelles. Neurofilaments are built from three intertwined protofibrils of apparent molecular weights [68 (L), 160 (M) and 200 (H) kDa] which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. Neurofilament 200 also known as Neurofilament heavy polypeptide (H-subunit), NF-H, NEFH or 200 kDa neurofilament protein, has an important function in mature axons that is not subserved by the two smaller neurofilament proteins.
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. Protect from prolonged exposure to light.
Disclaimer
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified from hybridoma cell culture
antibody product type	primary antibodies
clone	NE14, monoclonal
form	buffered aqueous solution
mol wt	200 kDa
species reactivity	guinea pig, rat, bovine, mouse, feline, chicken, human, pig
packaging	antibody small pack of 25 μL
concentration	~1 mg/mL
application(s)	immunohistochemistry: 1:200-1:400 (4-8 ug/mL) using enzyme treated formalin-fixed, paraffin-embedded rat Cerebellum sections
conjugate	FITC conjugate
shipped in	dry ice
storage temp.	−20°C
Gene Information	human ... NEFH(4744)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable