Anti-Nestin Antibody, clone 10C2

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

MAB5326

MAB5326-25UG

MAB5326-100UG

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.
Specificity
Reacts with human Nestin, with weak reactivity to monkey nestin. Not reactive with rodent nestin.
Epitope: human
Fusion protein.
Immunoblotting: 1:1,000-1:2,000 on denatured protein. Western blot: 1:1000-1:5000. Recognizes a 220-240kDA band on denaturing SDS gels. There is also a 60-64kDa band present in most lysates, the identity of this smaller fragment is unknown but it is not seen in lysates not containing human nestin protein.
WI-38 (ATCC# CCL75) can be used as a positive control. U251 cell line is also positive.
Immunocytochemistry: 1:200 on cells fixed in 4% paraformaldehyde and permeabilized with 0.02% Triton
Immunohistochemistry: paraformaldehyde is the preferred fixative: 1:200 on formalin fixed, paraffin embedded tissue sections. Use triton 0.02% in block only; dilution buffer 0.1% BSA; incubations 1-2 hours RT. Human fetal brain is suggested as a positive control. (CA Messam et al, Develop. Brain Res, 134:87-92, 2002; Gu, et al., Brain Res. 943:174-180, 2002). Filamentous staining in the cytoplasm of cells
Optimal working dilutions must be determined by end user.
Research Category
Stem Cell Research
Research Sub Category
Neural Stem Cells
This Anti-Nestin Antibody, clone 10C2 is validated for use in IC, IH, IH(P), WB for the detection of Nestin.
Target description
200-220 kDa
Replaces: AB5922
Physical form
Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified
Storage and Stability
Antibodies may be stored at 4°C or -20°C. Avoid repeated freeze/thaw cycles.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the containers cap.
Analysis Note
Control
Positive Control: WI-38 (ATCC# CCL75) or U251 cell lines
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified immunoglobulin
clone	10C2, monoclonal
species reactivity	human
should not react with	rat, mouse
packaging	antibody small pack of 25 µg
Торговая марка	Chemicon®
application(s)	immunocytochemistry: suitable","immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable","western blot: suitable
input	sample type neural stem cell(s) sample type epithelial cells
isotype	IgG1
NCBI accession no.	NM_006617.1,
UniProt accession no.	P48681,
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable