Anti-Nedd9 (C-terminal region) antibody produced in rabbit

NACRES:

NA.41

Кат. номер

N9539-25UL

N9539-200UL

NEDD9 (neural precursor cell expressed, developmentally down-regulated 9) is a non-catalytic multi-domain scaffolding protein. The gene contains retinoic acid response element (RARE) element in its promoter region, and is composed of seven exons. It encodes for a protein composed of 834 amino acids in humans, and contains an N-terminal SH3 (src homology 3) domain, followed by SD - substrate domain, SRR - serine rich region, FAT - focal adhesion targeting domain. Its C-terminal contains a DYDY motif (amino acids 628–631). This gene is expressed ubiquitously, and shows strong expression in lungs and lung cell lines. NEDD9 is a member of the Cas (Crk-associated substrate) family of scaffolding proteins.
NEDD9 gene is mapped to human chromosome 6p24.2.
Specificity
Anti-Nedd9 (C-terminal region) specifically recognizes human Nedd9.
Anti-Nedd9 (C-terminal region) antibody produced in rabbit may be used in immunoblotting and immunofluorescence.
Biochem/physiol Actions
NEDD9 (neural precursor cell expressed, developmentally down-regulated 9) functions in the assembly of oncogenic kinase complexes such as focal adhesion kinase (FAK), ABL, SRC, Aurora-A (AURKA). It, therefore, controls signaling pathways implicated in processes such as cell cycle, migration, adhesion, invasion and chemotaxis that are involved in for tumorigenesis and metastases. In melanoma, glioblastoma, colon cancer and head and neck squamous carcinoma, the overexpression of this protein results in enhanced epithelial to mesenchymal transition, migration, and metastasis.
Physical form
Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~120 kDa
species reactivity	human
packaging	antibody small pack of 25 µL
concentration	~1.0 mg/mL
application(s)	indirect immunofluorescence: 1.5-3.0 µg/mL using human HS68 cells.120","western blot: 1-2 µg/mL using Jurkat cell lysates
conjugate	unconjugated
UniProt accession no.	Q14511,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... NEDD9(4739) mouse ... Nedd9(18003) rat ... Nedd9(291044)

WGK Germany

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