Anti-Nanog Antibody, NT

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

AB5731-25UG

AB5731

NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency, i.e., these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).
NANOG may be useful in the immunohistochemical diagnosis of tumors. NANOG is expressed in germ cells of the fetus and in some germ cell tumors of the gonads and central nervous system (CNS).
Specificity
Antibody recognizes Nanog.
Synthetic peptide from mouse Nanog.
Epitope: N-terminus
Research Sub Category
Pluripotent & Early Differentiation
Western blotting:
0.5-1.0 µg/mL. The antibody recognizes a protein of ~35 kDa on NT2/D1 cell lysate. Additional bands of ~55 and ~130 kDa may be seen depending on sample and antibody dilution used.Optimal working dilutions must be determined by the end user.
Research Category
Stem Cell Research
Detect Nanog using this Anti-Nanog Antibody, N-terminus validated for use in WB.
Quality
Routinely evaluated by Western Blot on Embryonic Mouse Brain lysates.
Western Blot Analysis:
1:500 dilution of this lot detected NANOG on 10 µg of Embryonic Mouse Brain lysates
Target description
39 kDa (MW 34 kDa)
Physical form
Affinity purified
Purified rabbit polyclonal immunoglobulin in buffer containing liquid 0.1% sodium azide.
Storage and Stability
Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
Whole cell lysate from mouse embryonic stem cells.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity purified immunoglobulin
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	rat, mouse, human
packaging	antibody small pack of 25 µg
Торговая марка	Chemicon®
application(s)	western blot: suitable
input	sample type: mouse embryonic stem cell(s) sample type: human embryonic stem cell(s) sample type induced pluripotent stem cell(s)
isotype	IgG
NCBI accession no.	NM_024865.2,
UniProt accession no.	Q9H9S0,
shipped in	ambient
storage temp.	2-8°C