Anti-Na+/K+ ATPase -1 Antibody, clone C464.6

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

5-369

5-369-25UG

5-369-100UG

Na+/K+-ATPase (also known as the Na+/K+ pump or sodium-potassium pump) is an enzyme located in the plasma membrane (specifically an electrogenic transmembrane ATPase). It is found in the plasma membrane of virtually every human cell and is common to all cellular life. It helps maintain cell potential and regulate cellular volume.
In order to maintain the cell potential, cells must keep a low concentration of sodium ions and high levels of potassium ions within the cell (intracellular). Outside cells (extracellular), there are high concentrations of sodium and low concentrations of potassium, so diffusion occurs through ion channels in the plasma membrane. In order to keep the appropriate concentrations, the sodium-potassium pump pumps sodium out and potassium in through active transport.
Specificity
This antibody recognizes the α-1 subunit isoform of Na+/K+ ATPase.
Purified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.
Immunoprecipitation:
2 µg of a previous lot immuno-precipitated the -1 subunit from rat brain microsomal preparations.
Immunohistochemistry:
1-10 µg/mL of a previous lot of this antibody was used in immunohistochemistry on ice-cold methanol-fixed tissue.
1. Do not boil microsomes. Warming to 37C for 15 minutes is recommended.
2. Microsomes from some tissues may contain sodium pump subunits which are below the level of detection by this antibody.
3. Microsomes derived from tissues which are more difficult to prepare, such as smooth and skeletal muscle, frequently show non-specific cross-reactivity.
Detect Na+/K+ ATPase α-1 (sodium/potassium ATPase subunit alpha 1) using this Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6 validated for use in IC, IH, IP & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Quality
Routinely evaluated in western blot on rat brain microsomal preparations.
Western Blot Analysis:
0.05-0.1 µg/mL of this lot detected the -1 subunit in 20 µg of rat brain microsomal preparation (Catalog # 12-144). To reduce background, wash the blot with 0.05% Tween-20 in PBS for 15 minutes.
Target description
112 kDa
Physical form
Format: Purified
Protein G Chromatography
Purified mouse monoclonal IgG1 in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, 0.1% sodium azide. Frozen at
-20C.
Produced from a culture supernatant of a hybridoma produced by fusing Ag8 mouse myeloma cells with immunized Balb/c splenocytes. Clone C464.6 also called 6H.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Alpha 1 mRNA is present in all cell types examined.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	C464.6, monoclonal
species reactivity	rat, sheep, human, pig, monkey, Xenopus, mouse, rabbit, canine
packaging	antibody small pack of 25 µg
Торговая марка	Upstate®
application(s)	immunocytochemistry: suitable","immunohistochemistry: suitable","immunoprecipitation (IP): suitable","western blot: suitable
isotype	IgG1
NCBI accession no.	NM_001001586,
UniProt accession no.	P05023,
shipped in	dry ice
Gene Information	human ... ATP1A3(478)