Anti-Muscle Satellite Cells Antibody, clone SM C-2.6

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

MABT857

MABT857-25UG

Skeletal muscle satellite cells are quiescent mononucleated myogenic cells that reside between the sarcolemma and basement membrane of terminally-differentiated muscle fibers. They serve as a reserve population of cells with stem-like properties. The number of satellite cells are shown to be highest in postnatal muscle, but declines with age. In their quiescent stage, satellite cells are rounded and mononucleated, and do not express relevant amounts of myogenic proteins. Also, in their quiescent state they are shown to be c-kit-, Sca-1-, CD34+, and CD45-. Satellite cells can proliferate in response to injury and regenerate muscle and produce more satellite cells. Hence, they have been considered to be the only myogenic source for the maintenance of skeletal muscle. When muscle regeneration is initiated, satellite cells changes from their quiescent state to an active stage and begin to proliferative. They can proliferate and then either fuse with each other to form myotubes, which mature into myofibers, or they can fuse with damaged segments of muscle fibers. (Ref.: Fukuda, S et al. (2004). Exp. Cell Res. 296(2); 245-255; Morgan JE and Partridge, TA (2003). Int. J. Biochem. Cell Biol. 35(8); 1151 1156).
Specificity
Clone SM C-2.6 detects quiescent satellite cells from adult mouse muscle.
C2/4 myogenic cells.
Research Category
Cell Structure
Anti-Muscle Satellite Cells, clone SM C/2.6, Cat. No. MABT857, is a mouse monoclonal antibody that detects muscle satellite cells and has been tested for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Fluorescence Activated Cell Sorting (FACS).
Fluorescence Activated Cell Sorting (FACS) Analysis: A representative lot detected Muscle Satellite Cells in Fluorescence Activated Cell Sorting (FACS) applications (Urbani, L., et. al. (2012). PLoS One. 7(11):e49860).
Flow Cytometry Analysis: A representative lot detected Muscle Satellite Cells in Flow Cytometry applications (Ikemoto, M., et. al. (2007). Mol Ther. 15(12):2178-85; Fukada, S., et. al. (2004). Exp Cell Res. 296(2):245-55).
Immunohistochemistry Analysis: A representative lot detected Muscle Satellite Cells in Immunohistochemistry applications (Fukada, S., et. al. (2004). Exp Cell Res. 296(2):245-55; Yao, Y., et. al. (2016). Nat Commun. 7:11415).
Immunofluorescence Analysis: A representative lot detected Muscle Satellite Cells in Immunofluorescence applications (Fukada, S., et. al. (2004). Exp Cell Res. 296(2):245-55).
Quality
Evaluated by Flow Cytometry in C2C12 cells.
Flow Cytometry Analysis: 1 µg of this antibody detected Muscle Satellite Cells in a population of one million C2C12 cells.
Physical form
Format: Purified
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100,
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	SM/C-2.6, monoclonal
species reactivity	mouse
packaging	antibody small pack of 25 μg
application(s)	flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable
isotype	IgG1κ

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable