Anti-MRE11 Antibody, clone 15B8.1E7.6

eCl@ss:

32160702

Кат. номер

MABE1153-25UG

MABE1153

Double-strand break repair protein MRE11A (UniProt: Q61216; also known as MmMRE11A, Meiotic recombination 11 homolog 1, MRE11 homolog 1, Meiotic recombination 11 homolog A, MRE11 homolog A) is encoded by the Mrer11a (also known as Mre11) gene (Gene ID: 17535) in murine species. MRE11A a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. It localizes to discrete nuclear foci after treatment with genotoxic agents. MRE11A is a component of the MRN complex that possesses single-strand endonuclease activity and double-strand-specific 3-5 exonuclease activity. MRE11A forms a complex with the RAD50 homolog, which is required for non-homologous joining of DNA end. RAD50 may be required to bind DNA ends and hold them in close proximity, which facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. In conjunction with a DNA ligase, MRE11A is reported to promote the joining of non-complementary ends in vitro using short homologies near the ends of the DNA fragments.
Specificity
Clone 15B8.1E7.6 detects Double-strand break repair protein MRE11A in human and murine cells.
Recombinant fragment corresponding to 540 amino acids from murine Double-strand break repair protein MRE11A.
Anti-MRE11, clone 15B8.1E7.6 , Cat. No. MABE1153, is a highly specific armenian hamster monoclonal antibody that targets Double-strand break repair protein MRE11A and has been tested in Western Blotting.
Western Blotting Analysis: 1 µg/mL from a representative lot detected MRE11 in 10 µg of mouse embryo fibroblast cell lysate (MEFs).
Research Category
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blotting in Ramo cell lysate.
Western Blotting Analysis: 4 µg/mL of this antibody detected MRE11 in 10 µg of Ramo cell lysate.
Target description
~80 kDa observed; 80.22 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Replaces: PC388-100UG
Physical form
Purified armenian hamster monoclonal antibodyIgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	hamster (Armenian)
Quality Level	100
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	15B8.1E7.6, monoclonal
species reactivity	mouse, human
packaging	antibody small pack of 25 µg
application(s)	western blot: suitable
isotype	IgG
NCBI accession no.	NP_001297657,
UniProt accession no.	Q61216,
shipped in	ambient