Anti-Mouse CD86/B7-2 (GL-1) ColorWheel® Dye-Ready mAb CWA-1055-25UL

Specificity
Clone GL-1 is a rat monoclonal antibody that detects murine T-lymphocyte activation antigen CD86 (B7-2).
LPS-activated CBA/Ca mouse splenic B cells
Quality Control Testing:
Evaluated by Flow Cytometry in 3-day LPS-stimulated BALB/c mouse splenocytes.
Staining of one million untreated BALB/c mouse splenocytes and LPS- treated (2 µg/mL for 3 days) was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1055, Anti-Mouse CD86 (GL-1) Colorwheel® Dye-Ready mAb and Cat. No. CWD-PE Colorwheel® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of a PE-conjugated rat IgG2a isotype control.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Mouse CD86/B7-2 (GL-1) ColorWheel<® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any Colorwheel® antibody with any Colorwheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each Colorwheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise
Target description
T-lymphocyte activation antigen CD86 (UniProt: P42082; also known as Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1, CD86) is encoded by the cd86 gene (Gene ID: 12524) in murine species. CD86 is a homodimeric, single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 24-244), a transmembrane domain (aa 245-265), and a cytoplasmic domain (aa 266-309). It is constitutively expressed on interdigitating dendritic cells , Langerhans cells, memory B cells and germinal center B cells, and macrophages. It is rapidly upregulated on B cells following activation by cross-linking of the Ig receptor or by treatment with a variety of cytokines. Along with CD80 (B7-1) it provides costimulatory signals necessary for T cell activation and survival and displays about 25% homology with CD80. However, CD86 is more widely expressed and at higher levels than CD80. By binding to CD28 it provides costimulatory signals for T cell activation and survival and by binding to CTLA-4 (CD152) it plays a role in downregulating of T cell activity and immune regulation. Blocking the CD28-CD86 pathway is shown to induce cell death in myeloma cells and this is reported to be due to downregulation of integrins and interferon regulatory factor 4 (IRF4). (Ref.: Gavile, CM., et al. (2017). Blood Adv. 1(25); 2307-2319; Zheng, Y., et al. (2004). J. Immunol. 172(5); 2778-2784).
Physical form
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	200
biological source	rat
antibody form	purified antibody
antibody product type	primary antibodies
clone	GL-1, monoclonal
product line	ColorWheel®
form	lyophilized
mol wt	calculated mol wt 34.67 kDa
species reactivity	mouse
packaging	antibody small pack of 25 µL
application(s)	flow cytometry: suitable
isotype	IgG2a
epitope sequence	Extracellular domain
Protein ID accession no.	NP_062261.3 ,
UniProt accession no.	P42082,
shipped in	ambient
storage temp.	2-8°C
Gene Information	mouse ... Cd86(12524)