Anti-Mouse CD24 (M1/69) ColorWheel® Dye-Ready mAb CWA-1054-25UL

Specificity
Clone M1/69 is a rat monoclonal antibody that detects murine CD24.
Spleen cells from B 10 mice enriched for T lymphocytes and spleen cells treated with 1 pg/ml concanavalin.
Anti-Mouse CD24 (M1/69) ColorWheel<® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any Colorwheel® antibody with any Colorwheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each Colorwheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise
Quality Control Testing:
Evaluated by Flow Cytometry in C57Bl/6 mouse splenocytes.
Staining of one million mouse splenocytes was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1054, Anti-Mouse CD24 (M1/69) Colorwheel® Dye-Ready mAb and Cat. No. CWD-PE Colorwheel® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Rat IgG2b isotype control.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Signal transducer CD24 (UniProt: P24807; also known as Lymphocyte antigen 52, Ly-52, M1/69-J11D heat stable antigen,HSA, Nectadrin, R13-Ag, X62 heat stable antigen, CD24) is encoded by the Cd24 (also known as Cd24a, Ly-52) gene (Gene ID: 12484) in murine species. CD24 is a GPI-anchor protein without any intracellular signaling domain. It plays a pivotal role in differentiation of different cell types. Its expression is reported on hematopoietic cells, including B cells, T cells, neutrophils, dendritic cells, eosinophils, and macrophages. It is also expressed in several non-hematopoetic cells, including neuronal cells, keratinocytes, pancreatic cells, and several types of cancer cells. Its expression is shown to be higher in progenitor cells and metabolically active cells and to a lesser extent in terminally differentiated cells. In association with SIGLEC10, CD24 is reported to be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs), such as the high mobility group box protein 1 )HMGB1), HSP70, and HSP90. CD24 also plays a role in T cell survival. In the absence of CD24, homeostatic proliferation of T cells is markedly reduced, however immune-driven proliferation is less affected. (Ref.: Fang, X., et al. (2010). Cell. Mol. Immunol. 7(2); 100-103).
Physical form
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level	200
biological source	rat
antibody form	purified antibody
antibody product type	primary antibodies
clone	M1/69, monoclonal
product line	ColorWheel®
form	lyophilized
mol wt	calculated mol wt 7.80 kDa
species reactivity	mouse
packaging	antibody small pack of 25 µL
application(s)	flow cytometry: suitable
isotype	IgG2b
epitope sequence	Unknown
Protein ID accession no.	NP_001361159.1,
UniProt accession no.	P24807,
shipped in	ambient
storage temp.	2-8°C
Gene Information	mouse ... Cd24;Cd24a;Ly-52(12484)